The Experimental Research And Clinical Application In The Posterior Lamellar Corneal

Objective：To establish the lamellar keratoplasty model in rats, Compare thedifferent materials lamellar keratoplasty（Bowman layer+part of the stroma、Descemet+part of the stroma）and find out the clinical features and pathologicalconversion.Methods：The SD rats were randomly divided into two groups,54rats in each group.27Wistar rats were used as donor group. The two groups of rats were used forlamellar keratoplasty by Former lamellar and Post-lamellar. After surgery3,7, l4,28,42and56days by slit lamp and sodium fluorescein staining of corneal epithelium,killed the experimental group and control group rats9, take the cornea preparation ofHE stained, transmissionelectron microscopy specimens and immunofluorescencedetection.Results：108SD rats anesthesia excessive dead14, graft infection7. Slit lampobservation: the experimental group and the control group of early postoperativecorneal graft opacity were seen in edema, late two group corneal opacity edemagradually reduced to disappear, RI curve chart almost overlapped, the peak value andthe overall curve trend is largely the same, the overall RI index declined. Sodiumfluorescein dye: two groups of sodium fluorescein staining scope about the same time.Photoshop drawing software aided analysis of corneal epithelial growth area wasobserved in two of patients at different time points after corneal epithelial crawl areadifference is very small. On RI and Fls score data and epithelial growth area, take twosamples t test, a=0.05, p<0.05data was statistically significant, calculated at eachtime point p>0.05, the differences were not statistically significant. HE staining: lightmicroscopic observation after operation in the two groups were from the earlyepithelial growth not typical to the late skin structure are long out, form close tonormal epithelial cells. Transmission electron microscopic observation: two groups ofearly corneal epithelial cell microvilli are sparse, later to five layers of epithelial cellswere long, dense microvilli growth. At the same time were observed in the corneal epithelial basement membrane and after the elastic layer connected with a gap, and istightly connected with Bowman layer. K12immunofluorescence detection: the twogroups after immunofluorescence staining showed epithelial cells from early to latestrong weak fluorescence fluorescence.Conclusions：1, first established rat allogeneic sutureless front, rear LKP animalmodel.2, through the detection of indexes, were observed in the two groups at different timepoints epithelial crawl rate, size and morphology of roughly the same, confirmed thebackboard can also serve as corneal epithelial cell growth plate carrier, for furtherapplications provide experimental and theoretical basis.3, observed by transmission electron microscope, after before after LKP cornealepithelial migration ultrastructural differences, for follow-up research work providesan important reference.4, swine fibrin adhesive applied to rat lamellar keratoplasty suture operation, to nopurpose, for its further application in sutureless corneal transplantation operationprovided experimental basis.