The Effect Of Hydroxycamptothecin On NF-κB Expression Of Human Hypertrophic Sear Fibroblasts

Hypertrophic scar (HS) is severely affected people，s appearance and function, reducing the quality of life.So researchers have long tried to use a varietyof solution for the pathological scar, including surgery, drugs, radiation, pressure, and so on.But there is still not satisfactory curative effect.On the base of the previous studies of10-hydroxycamptothecin on human hypertrophic scar fibroblasts,we continue to explore the mechanism of HCPT on NF-κB expressionof human hypertrophic scar fibroblasts.Objective：In order to understand the mechanism of HCPT on treat scar, this studyobserved the effects of HCPT on NF-κB expression of cell transcription factor.Methods：HS obtained from patients who received plastic surgery, and then hypertrophicscar fibroblasts (HSFB) were cultured in vitro using tissue culture method. HSFB wasintervened by HCPT with various concentrations (125、250、500ng/ml) for24hours,then the expression of NF-κB p65、IκBα mRNA was measured with RT－PCR andNF-κB p65、IκBα protein was detected with western blot method.Results：(1) Compared with control groups0.104±0.032,0.974±0.039, the expression ofNF-κB p65、 IκBαmRNA in experiment groups is0.268±0.032、0.870±0.051，0.562±0.051、0.762±0.067，0.790±0.038、0.664±0.086with various concentrations(125、250、500ng/ml). There is significant difference between control groups andexperiment groups (P＜0.05). These is indicated that hydroxycamptothecin couldsignificantly increase the expression of NF-κB p65mRNA and reduce theexpression of IκBαmRNA in human hypertrophic scar fibroblasts，especially when itsconcentration at500ng/mL.(2) Compared with control groups0.271±0.114，1.090±0.017，the expression ofNF-κB p65、 IκBαprotein in experiment groups is0.455±0.012、1.060±0.033，0.593±0.015、0.736±0.016，0.765±0.023、0.555±0.021with various concentrations (125、250、500ng/ml). There is significant difference between control groups andexperiment groups (P＜0.05). These is indicated that hydroxycamptothecin couldsignificantly increase the expression of NF-κB p65protein and reduce the expressionof IκBαprotein in human hypertrophic scar fibroblasts， especially when itsconcentration at500ng/mL.Conclusion：Hydroxycamptothecin could reduce the expression of IκBαmRNA andprotein,which to enhanced the expression of NF-κB p65mRNA and protein, whichhas a role in the treatment of hypertrophic scars.