The Expression And Significance Of CDC4and Cycline In Gastric Cancer

BackgroundGastric tumour is the fourth most common malignancy in the world and second in terms of cancerrelated death, Gastric tumour is deem to result from a combination of environmental factors and specific genetic alterations。CDC4, encoding a ubiquitin ligase, maps to4q31.2and has been implicated as a cancer suppressor gene and therapeutic target in many cancer categories. CDC4gene Mutates in colonic adenomas,and the frequent losses on4q31described in gastric tumour prompt speculation about the part of CDC4in gastric carcinogenesis。PurposeTo investigate the expression of CDC4and Cyclin E in gastric cancer and elucidate the potential relationship between their expression and clinical pathological characteristics.MethodsSemiquantitative reverse transcription polymerase chain reaction (RT-PCR) was determined the mRNA expression of CDC4and Cyclin E in part of random selecting gastric cancer samples and corresponding adjacent normal mucosal tissues; Immunohistochemisty was used to detecte the protein expression of CDC4and Cyclin E in60specimens of gastric cancers and corresponding adjacent normal mucosal tissues. The expression of CDC4、Cyclin E and the clinical pathological characteristics were analyzed.ResultsThe protein expression of CDC4in gastric cancer tissues were significant lower than that in adjacent normal mucosal tissues (P<0.05),whereas the protein expression of Cyclin E in gastric cancer tissues were significant higher than that in adjacent normal mucosal tissues (P<0.01). The protein and mRNA expression of CDC4、Cyclin E were correlated with differentiation、TNM staging、lymph node metastasis、 infiltration.There was a significant negative correlation between CDC4and Cyclin E at the mRNA expression level.ConclusionCyclin E overexpression is likely to be the CDC4/FBXW7loss. It suggests that the loss of CDC4maybe become a valuable marker for assessing the diagnosis and treatment and the prognosis of gastric cancer.