| Objectives: To explore the sensitivity effects and the potentialmechanism on pEGFPN1-dnEGFR transfection combined with radio-therapy in human gastric carcinoma cell lines, SGC-7901and NCI-N87.Methods: pEGFPN1-dnEGFR vector and the empty vector withoutdnEGFR gene were transfected into SGC-7901and NCI-N87cells bylipofection respectively, and the stable cell lines were obtained by G418screening. The proliferation of human gastric carcinoma cells, SGC-7901and NCI-N87, which were transfected pEGFPN1-dnEGFR and conductedradiation treatment, was determind by MTT assay. The mRNA expressionsof Bax and Cyclin D1were detected by reverse transcription PCR (RT-PCR)in each group. Protein expressions of Bax and Cyclin D1in cells weredetected by Western blot.Results: The VIs of pEGFPN1-dnEGFR transfection combinedradiotherapy cell groups were decreased significantly compared with thecontrol group, while the Bax mRNA expression was increased in thecontrol group. Western blot results showed that Cyclin D1proteinexpression in pEGFPN1-dnEGFR transfection combined with cell group was reduced compared with the control, and the expression of Bax proteinwas increased significantly.Conclusion: pEGFPN1-dnEGFR could improve radiotherapysensitivity of gastric carcinoma cells. |
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