Contrast Echocardiography During Adenosine Stress For Myocardial Microcirculation Perfusion On Canine Using Aortic Root Injections Of Microbubble

PART1EVALUATION OF MYOCARDIAL MICROCIRCULATION PERFUSION IN DOGS BY ADENOSINE STRESS MYOCARDIAL CONTRAST ECHOCARDIOGRAPHYObjective1. Establishment of a canine model of acute myocardial infarction;2. To investigate the feasibility of resting and adenosine stress real-time myocardial contrast echocardiography (MCE) on quantitative detection myocardial perfusion abnormalities in different graded coronary steonosis of canine. Methods1. Twenty-eight mongrel dogs were enrolled and3of them were selected as normal group. The other25dogs were in experimental group. Different degrees of left anterior descending artery stenosis were established by median thoracotomy, pericardial cradle formation, ligation of the left anterior descending coronary artery Pericardium, muscle, subcutaneous and skin tissue were then sutured according to the order. Wound dressing, anti-inflammatory treatment were used for three days.2. Experimental group was divided into3groups according to the results of coronary angiography:group I (50%-75%) stenosis,(75%-99%) stenosis group Ⅱ, group III in100%obstruction. On the experimental group and normal control group, the rest and adenosine stress myocardial contrast echocardiography was performed, and followed TTC, evans blue staining. Quantitative analysis was performed by Qlab8.0software to describe regional myocardial micro bubble filling curve, from which the A, β and A×β were obtained. Results At rest, A, β, A×β declined along with the higher stenosis grades, differences were found in normal groups and experimental groups II and III (P<0.05, P<0.01), there was no difference found between group I and normal group (P＞0.05). At stress, A, P, A×β increased in group I and normal group while declined in group II and III. The stained preparation reflected the ischemic and infarcted regions. Conclusions MCE can quantitatively evaluate the myocardial blood flow in different degree ischemic area. PART2CONTRAST ECHOCARDIOGRAPHY DURING ADENOSINE STRESS FOR MYOCARDIAL PERFUSION USING AORTIC ROOT INJECTIONS OF MICROBUBBLEObjective The aim of this study was to evaluate the myocardial contrast echocardiography during adenosine stress using aortic root injections with microbubble to assess myocardial perfusion. Methods24healthy mongrel dogs were enrolled, the left anterior descending artery was occluded for120min followed by60min reperfusion, myocardial contrast echocardiography using aortic root injections with microbubbles were used during the time of occluded, reperfusion and adenosine stress.5dogs were chosen for SPECT, the rest and adenosine stress SPECT were used for myocardial perfusion evaluation. TTC and evans blue staining were used after the ultrasound or nuclear testing. Qlab8.1were used to analysis the images in order to evaluate myocardial perfusion. The myocardial segments of ischemia and infarction checking results of SPECT, MCE and TTC-EB were calculated, and the specificity, sensitivity were figured. Results The specificity of myocardial contrast echocardiography with aortic root injection of microbubble/SPECT,/TTC-Evans blue dye staining were64.2%,71.2%, the sensitivity was81.5,72.4%,,respectively. Conclusions Myocardial contrast echocardiography during adenosine stress using aortic root injections with microbubble could accurately assess myocardial perfusion.