Atorvastatin Reduces Myocardial Ischemia Reperfusion Injury In Rats Via CREB Activation

Objective To observe the effect of pretreatment with atorvastatin onthe expression of p-CREB and the degree of ischemia reperfusionmyocardial cell apoptosis in rats,to find the relationship between atorvastatinwith myocardial ischemia reperfusion injury.Methods Ligating the left-anterior-descending coronary artery tostructure the model of myocardial ischemia reperfusion injury in rats.48adult male SD rats were randomly divided into shame-operated group(shamgroup), ischemia reperfusion injury group(IR group), atorvastatin group(ATgroup) and Atorvastatin+LY294002group(AT+LY group).rats were subjectto30min of left-anterior-descending coronary artery (LAD) occlusion and120min reperfusion.Monitored the cardiac hemodynamic of rats after120min reperfusion,myocardial pathological structure in the infracted andnon-infarcted zone were observed with histopathological methods,detectedthe expression of p-CREB and p-Akt protein in myocardium by Westernblotting and immunohistochemistry, the apoptosis cells were detected byTUNEL. Result Compared with sham group,left ventricular systolicpressure(LVSP)、 maximal ascending velocity of the left ventricularpressure(＋LVdp∕dtmax) and maximal descending velocity of the leftventricular pressure(－LVdp∕dtmax) of other three groups weredepressing,the left ventricular end-diastolic pressure(LVEDP) wasincreasing(P＜0.05). Compared with IR group,the LVSP and±LVdp∕dtmaxof AT group and AT+LY group were increasing,the LVEDPwas depressing(P＜0.05). Between AT group with AT+LY group, thecardiac hemodynamic had no difference（P＞0.05）.(2) Compared with shamgroup,major anomalies of myocardial pathological structure in other groups;Compared with IR group,noticeable improvements of myocardialpathological structure in AT group and AT+LY group.(3) Compared withsham group,the number of apotosis cells of other three groups wereincressing(P＜0.05). Compared with IR group, the number of apotosis cellsof AT group and AT+LY group were depressing(P＜0.05).There was nodifference between AT group with AT+LY group （P＞0.05）.(4)Incomparison with the sham group, the expression of p-CREB and p-Aktprotein in myocardium of IR group was no difference（P＞0.05）.Comparedwith IR group, the expression of p-CREB and p-Akt protein in myocardiumof AT group and AT+LY group was incressing(P＜0.05). The expression ofp-CREB and p-Akt protein in myocardium of AT group was more thanAT+LY group(P＜0.05).(5)The results between Western blotting with immunohistochemistry were the same.Conclusion Atorvastatin can reduce the degree of myocardialischemia reperfusion injury and protect the heart.Apoptosis of cells play animportant role in myocardial ischemia reperfusion injury. Atorvastatin canactivate the PI3K/AKT pathway to increase p-CREB,apoptosis can berestained,then,to inhibit myocardial ischemia reperfusion injury.