| Objective To research the anastomosis by using aortic rings are better than theordinary suture anastomosis in the operation time and anastomotic bleeding rate.Methods Select15dogs, these dogs weight amount15-20kg, age2-5years old,in good health.5dogs were ligated aortic with aortic ring as the test group and5dogs whose aorta anastomosis was sutured in the tradition way were as the controlgroup. Another5dogs were used in preliminary experiment (based onimproving the experimental design of aortic rings,we have carried out5preliminaryexperiments). All dogs were carried out tracheal intubation under generalanesthesia,and then the chest were opened in the centre to establish cardiopulmonarybypass in which we use cold crystalloid cardioplegia. Record cardiopulmonary bypasstime, aortic clamp time and the time of vascular anastomosis. In the test group, wehave applied the aortic rings. The anastomosis of aortic rings is one of a method ofmechanical vascular anastomosis. After we repeatedly improved ring design andsurgical procedures in4preliminary experiments, the initial shape is made. The ringanastomosis device is divided three parts: the proximal ring, artificial blood vessels,the distal ring. The rings of The animal experiments were made of stainlesssteel.There is2U gap (their center is separated by rings1/3circumference) on Theheart end of The proximal ring, and the edge of the ring has number of holes; thedistal ring end has the wide groove. The distal ring has only one wide groove. Itsanastomosis principle: before the experiment,we advance to set rings into artificialblood vessel of the same caliber;and we take dacron line through the holes in theproximal ring and No.7black silk ligated in the wide groove to fix the ring toartificial blood vessel while the distal ring fixed directly with No.7black silkline.the artificial blood vessel’s part near the two U-shaped gap is removed.In thecase experiments, firstly we take prefabricated component’s proximal end two two U-shaped gap toward the dog’s two coronary arteries, and then take dacron suture andline through holes of ring to sew into the aortic root (to sew into the aortic annulus asfar as possible) to fix it.The dacron line is tied outside the aortic after it pass throughthe aortic wall,when we place the proximal end of the component in theaortic;Thirdly use the7th black silk line to tie fixing components.As the same way thedistal end of the component is connected to the aortic. As follows:The test group (rings anastomoses): when experimental canines occur to cardiacarrest, we cut the aorta at1cm above the junction of the aortic sinus and theascending aorta,and then Use the aforementioned method. Observe if the ligationhappen to bleed after the heart resuscited. And record The CPB time, aortic clampingtime and anastomosis time of ligation, the heart rate and the mean artery pressurewhen the cardiopulmonary bypass machine has been stoped working.The controlgroup (traditional anastomosis): when experimental canines occur to cardiac arrest,we cut off part of the ascending aorta, and sew to connect aortic with artificial bloodvessel. Observe if the Anastomotic happen to bleed after the heart resuscited. Andrecord the time, the heart rate and the mean artery pressure.Results Test group:the CPB time:9,8,8,7,6min,; the aortic clamping time:4.5,4,4,3.5,3min; the aortic ligation anastomosis:4,3.5,3.5,32.5min; the heartrate:160/min,179/min,170/min,172/min,165/min； the mean arterypressure:106,100,108,112,104mmHg.Control group: the CPB time:17,16,17,18,18min; the aortic cross-clamping time:8.5,8,8.5,9,9min; the aortic anastomosis time:8,7.5,8,8.5,8min; the heart rate:160/min,179/min,170/min,172/min,165/min；themean artery pressure:102,108,107,103,105mmHg. Open cycle of canine heart weresuccessful resuscitation.Conclusion The aortic rings ligation method take the shorter operating time,witha low incidence of anastomotic bleeding, significantly better than the aortic ordinarysewing anastomosis. The aortic rings had no effect on the coronary perfusion. |
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