| Background Fracture healing is a complex process after a fracture in which a variety of cells and growth factors are involved. Fracture patients are prevalent in the clinic, and fracture patients with diabetes are common. The delayed union is a thorny problem to be solved which is also a relatively common clinical problems faced by clinicians. In our previous experiment on Wistar rats,we found that the low content of IGF-1in partial callus tissue and serum is one of the reasons resulting in the delayed union in fracture healing process with diabetes. With the development of gene engineering and stem cell technique, bone marrow stromal cells become crucial in treating and studying various orthopaedic diseases and the complications. In our previous experiment, we also found that it is feasible for BMSC to be transfected by IGF-1gene that was incorporated into adenovirus under high glucose environment. After gene transfection, the IGF-1can be stably expressed in the transfected BMSCs. IGF-1can even enhance the proliferation of BMSCs cultured in vitro. But does the phenomenon exists in vivo? To confirm this phenomenon, we designed the present experiment in which we transplanted the BMSCs transfected by IGF-1gene in vitrop into the fracture area on Wistar rats to authenticate the possibility that they can promote the fracture healing with diabetes. In the present experiment, we measured the content of IGF—1in serum and callus during the fracture healing process at different times.Objective To observe the effect of transplantation of Bone Marrow Mesenchymal Stem Cells (BMSCs) transfected by insulin-like growth factor-1(IGF-1) gene on fracture healing of diabetic rats.Methods Fifty male Wistar rats were divided randomly into control group and transfection group. Streptozotocin was injected into celiac to induce diabetes model and all suffered right tibia fracture. Ad-IGF-1transfected rat BMSCs under high glucose condition in vitro. After transplantation of BMSCs transfected by IGF-1, X-ray photographs were taken at1,2,3,4,6weeks, and the callus was subjected to HE staining.The levels of IGF-1of the callus and serum were detected by immunohistochemical method and ELISA respectively.Results There were significant differences in the expression of the IGF-1gene between the two groups.At4weeks,the gray scales of IGF-1were103+5in transfection group and109±4in control group (P<0.05). The serum concentrations of IGF-1at4weeks were (668.80±8.07) ng/L in transfection group and (569.20±9.31) ng/L in control group (P<0.01)Conclusion The transplantation of BMSCs transfected by IGF-1gene can promote the fracture healing of diabetic rats. |
PDF Full Text Request