The Mechanisms Of Ilk Signal Pathway On Renal Interstitial Fibrosis In Uuo Rats And Treatment Of Zhenwu Decoction

Objective:1.Using the Unilateral ureteral obstruction (UUO) method to copy rats renal fibrosis model, with the urine poisonous clear particles for comparison, dynamic observation of UUO Zhenwu decoction rat models of kidney function, the change of ideology and renal tubular damage and renal fibrosis degree.2.Dynamic observation UUO rat models of the proteins of fibronectin (FN), matrix metallo proteinases9(MMP-9), angiotensin Ⅱ (Ang-Ⅱ) and integrin linked kinase (ILK) protein expression, this paper discusses the rats with renal fibrosis of the relationship.3.Discusses Zhenwu decoction against antagonism rat renal fibrosis role mechanism, so as to help clinical.Methods:150SPF health male wistar rats, weight (180-200) g, were divided into control group and made module, which Control group21,129made module; Made module rats are divided into groups of five, is model group, and the Zhenwu decoction low dose group, in high doses, dose group of group and urine poisonous clear control; Using the method of unilateral ureter ligation copy rats renal fibrosis model, Control group group cut and free the left only abdominal ureteral, do not do the ureteral ligation, from postoperative day one to medicine, take one time every irrigation, respectively, serve for irrigation2weeks, three weeks and4weeks; Control group group and the model group take5ml distilled water irrigation, Zhenwu decoction low dose group take5ml Zhenwu decoction irrigation low dose decocting (0.28g/ml), take5ml dose group of irrigation in dose decocting (0.56g/ml), high dose group take5ml high dose irrigation decocting (1.12g/ml), urine poisonous clear control group take5ml urine filling poisonous clear solution (0.15g/ml); After two weeks, respectively in3weeks and four weeks ago one day rat as for metabolic cage collect urine testing24h urine protein quantitative; Rats take femoral artery blood, serum separation, divided into two, a serum creatinine, used in the detection of urea nitrogen content, another using ELISA test Ang-Ⅱ and ILK protein expression; Take ligation side kidney tissues on the10%of formaldehyde fixed, paraffin wax fixed, HE used the method of dyeing detection kidney the morphology of the change and renal tubular damage and renal fibrosis degree of evaluation, the immunohistochemistry methods detection kidney tissues FN and MMP-9in the protein expression.Results:1.Zhenwu decoction to UUO rat of protection. The24h urine protein rats quantitative results show that no statistically significant (P>0.05); Zhenwu decoction each dose group can reduce UUO model of rats serum creatinine, urea nitrogen are statistically significant (P<0.05, P<0.01), the best dose group of urine, and poisonous clear control group quite.2.Zhenwu decoction to UUO rats kidney organization form and FN and MMP-9the protein expression. Zhenwu decoction reduced the UUO model of rats pathological damage and reduce the renal tubular damage and renal fibrosis degree, inhibit the renal tissue FN and MMP-9protein expression, a statistically significant (P<0.05, P<0.01) urine poisonous clear in the control group and dose group of quite.3.Zhenwu decoction rats to UUO Ang-Ⅱ and ILK signaling pathways protein expression. Zhenwu decoction UUO can reduce rat models of serum Ang-Ⅱ and ILK protein content, a statistically significant (P<0.05, P<0.01), Zhenwu decoction dose group of Ⅱ Ang-and ILK protein content, and the lowest urine poisonous clear control group content quite.Conclusion:1.Zhenwu decoction UUO model of rats have protective effect, can reduce serum creatinine, urea nitrogen content.2.Zhenwu decoction that can reduce UUO model of rats kidney pathological damage, reduce renal tubular damage, antagonist renal fibrosis.3.Zhenwu decoction inhibit UUO rat models of serum Ang-Ⅱ and ILK protein and kidney tissues FN and MMP-9of protein expression.4.Zhenwu decoction Ang-Ⅱ cut through the protein expression, thus inhibiting protein expression ILK, then to lower FN and MMP-9of the protein content, which affects the transmission of signaling pathways, slow down the development of renal fibrosis, speculate it may be Zhenwu decoction prevention renal fibrosis mechanism.5.Zhenwu decoction low, medium and high dose group and control group were poisonous clear urine to a certain extent protection renal function, reduce renal tubular damage and renal fibrosis degree, Zhenwu decoction dose of best effect to the clinical treatment of renal fibrosis reference.