Research On The Relationship Of Property-Effect Of Bitter-Cool Phellodendri Chinensis Cortex Radix Scutellariae Based On Biomarkers And Their Changed Rule

Objective:To the whole of the fever syndrome metabolic network and its biomarkers as the breakthrough point, research of syndrome, Phellodendri Chinensis Cortex fever intervention effect, thus illustrating fever syndrome models of Phellodendri Chinensis Cortex and the experience with the relevance of medicinal effect, initially revealed the scientific connotation of traditional Chinese medicinal properties.Methods:The " fever syndrome " and its variation of biomarkers as a starting point, application of metabonomics methods, such as research using UPLC-MS technical means, through the study of biological body fluids (urine) change at different time points changes in metabolic spectrum to determine the course of medicinal effects, mechanisms, targets and biomarkers. Model by studying the fever syndrome (DNP and DY induced) and Phellodendri Chinensis Cortex in vivo biological markers after the intervention and its variation, intervention embodied in the process of property-effect relationships, summed up the changing role of law Phellodendri Chinensis Cortex treat fever syndrome, and then elucidate the property-effect relationship.Results:1, To macroeconomic signs and metabolomics method to evaluation model, copied successfully DNP induction of fever syndrome animal model. At the same time, determine the fever syndrome can be used to characterize the occurrence, the development of positive ions detection mode61biomarkers and6in the structure of biomarkers, respectively, formyl-5-kynurenine hydroxyl amine, L-2-amino adipic acid, L-phenylalanyl-L-hydroxy acid Prolinol acid, and tryptophan, lysine, proline and phenylalanine and other metabolites associated; Phellodendri Chinensis Cortex high, low dose group of26,21respectively potential biomakers has callback trend.2, To macroeconomic signs and metabolomics method to evaluation model, copied successfully DY induction of fever syndrome animal model. At the same time, determine the fever syndrome can be used to characterize the occurrence, the development of positive ions detection mode and a potential biomarkers, and1in the biomarkers structure:L-phenylalanyl-L-hydroxy acid Prolinol acid associated metabolism; The intervention of Phellodendri Chinensis Cortex does not play a significant role:Phellodendri Chinensis Cortex high, low dose groups were to8,7of the potential biomarkers has callback trend.3, Determine the two groups of model of common biomarkers8, and can be used as Fever syndrome evaluation index. More DNP fever syndrome and DY fever syndrome, proof that the two kinds of fever syndrome in metabonomics level differences, and Phellodendri Chinensis Cortex for two kinds of fever syndrome different intervention role.Conclutions:1, Copied successfully two fever syndrome, and determine the characterization of biomarkers of fever syndrome.2, Phellodendri Chinensis Cortex, to fever syndrome biomarkers intervention proves, high dose group slightly better than the low dose group, no obvious difference.3, Verify the traditional Chinese medicine at Phellodendri Chinensis Cortex utility, and from the body of the overall and biomarkers metabolites the changes in the structure of Phellodendri Chinensis Cortex intervention fever syndrome process.4, Phellodendri Chinensis Cortex the induction of endogenous amino acid metabolism disorders has good intervention role.5, By comparison, the two groups to determine the model to biomarkers8, and can be used as evaluation indexes of fever syndrome. And Phellodendri Chinensis Cortex to DNP fever syndrome intervention role of DY fever syndrome than the intervention of the role.