New HDAC Inhibitor CC1007Inhibit Proliferation,Migration, And Invasion Of Cervical Cancer Cells In Vitro

Purpose To investigate the effect of histone deacetylase inhibitors(HDACI) CC1007, on proliferation, clone formation, migration and invasion of human cervical carcinoma cell lines HeLa, Caski and C33a in vitro.Experimental Design1. Human cervical carcinoma cell lines:HeLa,Caski and C33a treated with different concentration of CC1007in logarithmic growth phase for48hours,then cell proliferation inhibition were measured by The CellTiter-Glo(?) Luminescent Cell Viability Assay, make the50%inhibiting concentration (IC50) of cells and the concentration-inhibition rate curve. Experimental repeated three times.2. HeLa,Caski and C33a treated with different concentration of CC1007in logarithmic growth phase for14days,then Cell colony formation inhition were measured by Flat cloning experiment. Experimental repeated three times.3. The effect of CC1007on migration ability of HeLa,Caski and C33a meatured by transwell chamber migration models.4. The effect of CC1007on invasion ability of HeLa,Caski and C33a meatured by transwell chamber invasion models.Results 1. Cell proliferation measured by The CellTiter-Glo(?) Luminescent Cell Viability Assay. HeLa, Caski and C33a treated with10μmol/L,20μmol/L30μmol/L,40μmol/L of CC1007for48hours, respectively. and the proliferation inhibition rate of HeLa were:39.33±0.93%,70.6±0.678%,86.93±0.55%,91.08±0.15%; the proliferation inhibition rate of Caski were:26.94±2.95%,59.61±0.33%,91.61±2.4%,98.79±0.14%;the proliferation inhibition rate of C33a were:67.34±1.54%,83.12±0.19%,90.72±0.19%,92.11±0.64%.The inhibition rate was in a dose dependent manner.Aftet treated with CC1007for48hours, the IC50of HeLa were and12.53μmol/L, the IC50of Caski were15.08μmol/L and the IC50of HeLa were5.36μmol/L.2. Cell colony formation inhition were measured by Flat cloning experiment. HeLa, Caski and C33a treated with2μmol/L,4μmol/L of CC1007for14days, respectively. The colony formation inhition rate of HeLa were:43.54±2.30%,3.61±1.19%;the clony formation inhition rate of Caski were:44.31±2.60%,3.09±0.61%; the clony formation inhition rate of C33a were:33.46±2.51%、2.92±0.84%.The inhibition rate was in a dose dependent manner.3. The effect of CC1007on migration ability of HeLa,Caski and C33a meatured by transwell chamber migration models.t. HeLa, Caski and C33a treated with20μmol/L,40μmol/L of CC1007for12hours, respectively.The number of Hela migrated to the surface of the lower chamber of Transwell chambers were:255.67±12.5,131±6.93; The number of Caski migrated to the surface of the lower chamber of Transwell chambers were:145±9.64,77.67±0.58; The number of C33a migrated to the surface of the lower chamber of Transwell chambers were:192.67±23.35个、128.33±35.57.The inhibition of migration was in a dose dependent manner.4. The effect of CC1007on invasion ability of HeLa,Caski and C33a meatured by transwell chamber migration models.t. HeLa, Caski and C33a treated with20μmol/L,40μmol/L of CC1007for12hours, respectively.The number of Hela invased to the surface of the lower chamber of Transwell chambers were:240.67±23.71,130.33±22.9; The number of Caski invased to the surface of the lower chamber of Transwell chambers were:160±4.36,76.33±5.03; The number of C33a invased to the surface of the lower chamber of Transwell chambers were:32.33±5.13,13.67±1.15.The inhibition of invasion was in a dose dependent manner.ConclusionCC1007could inhibit the proliferation, colony formation, migration and invasion of human cervical carcinoma cell lines obviously, in dose dependent manner.