Study Of Protective Effects On Endodermis Progenitor Cells To Acute Kidney Injury In Mice

Objective:To explore the homing of endothelial progenitor cells. To discover the possibility of endothelial progenitor cells differentiation into kidney tubules epithelium cells. To explore the stem cells can convert into blood vessels epithelial cells. To identify the genetic vascular epithelial cells. To discuss the self-recovery of acute injured kidney has the promotive effect by the treatment with endothelia progenitor cells.Method:Female mice (n=30), randomly divided into three groups which were sham group, control group and experimental group (n=10). In order to establish acute kidney injury animal models, to anaesthetize the mice with4%hydration chlorine aldehyde solution intraperitoneal injection, on the left back of belly made surgical skin incision, then find out the kidney, clip renal artery, observe the color of the kidney. When it darkle, keep30minutes, then restore blood flow. For experiments involving cell infusion after acute kidney injury, undifferentiated endothelial progenitor cells were implanted into renal capsule of the mice. The distribution of labeled endothelial progenitor cells in kidney was observed in living mice by bioluminescence imaging techniques. After operation mice were sacrificed on the3rd day and the14th day respectively, the changes of renal function and pathology were observed. Cell proliferation was conformed by BrdU assay and were detected by immunohistochemisty. The proteins expression of CD31were detected by immunofluorescence technique. The protein expression of TNF-a and IL-1β were detected by immunohistochemisty.Result:EPC in the ischemia side of kidney were observed through bioluminescence imaging techniques. Immunofluorescence results showed BrdU was positive in the injury kidney(73.83±4.58). Immediate transplanted EPC can survive in kidney tissue of the injured kidney. The result of BrdU has a statistical significant difference between the three groups. In the experimental group, the green fluorescence increased apparently. Immunofluorescence results showed that significant expression of CD31was postitive in the injury kidney(32.40±1.78), the experiment group were more than those in the control group(P<0.05). The increment of cells was detectabled after the endothelial progenitor cell infusion. The epithelial blood vessels of renewal resided and capillary density increment in injury kidney. The transference of EPC from the mice has promoted endotheliocyte regeneration effectively. Inflammatory factor immunohistochemical method with TNF-α、 IL-1p showed inflammation reaction obviously abatement The transplantation of endothelial progenitor cells can show up-regulation of inhibition to the phlogocyte. Analyses the degree of kidney injury according to data of serum creatinine and urea nitrogen. The experimental group is lower than control group, it has differences statistics(P<0.05). The endothelial progenitor cells infusion ameliorated the decline of renal function. Conclusion:Cultured endothelial progenitor cells could repopulate in the acute kidney injury, contributing to the repair of the acute kidney injury of the mice. Endothelial progenitor cells promote kidney tissue repair via direct differentiation. Endothelial progenitor cells promote the repair of the acute kidney injury of the mice.