| Objective: to observe the effect of MP on proliferation andmigration of endogenous neural stem cells after SCI in adult rats.Methods:①90adult female healthy SD rats were randomly divided into A, B, C,three groups: group A, methylprednisolone group,30; group B,physiological salt and water group,30; group C, the blank operationcontrol group,30.②A, BGroup rats were the WD into the legal systemin SD rats T10plane completely paraplegic model. MP concentration of0.9%NS configured to a1mg/ml solution of A rats via the tail vein within1hour slow bolus injection of MP (30mg/kg), followed by23hourscalculated in accordance with5.4mg/(kg.h), the average three times,each time interval the same time, the slow tail vein injection.Group B,injection of normal saline in the same way. The same parts of the group Claminectomy. Each group of rats after spinal cord injury within2hours ofBrdu (5-bromo-2-deoxy uridine) in accordance with the dose ofintraperitoneal injection of50mg/kg for10days.③After themodel-making14d,21d,28d each group of rats from each of10, respectively, remove the injured spinal cord segments, usingimmunohistochemical methods to detect Brdu labeling of endogenousneural stem cell proliferation and migration; BBB movement rating scorewere detected in rat lower limb motor function.Result:①Spinal cordslices of the two groups of animals showed a large number of Brdu-positive cells exist.②Methylprednisolone group of Brdu in spinal cordslices at each time point (5-bromo-2-deoxyuridine)-positive cells thanthe saline group was significantly reduced.③Two groups of rat spinalcord slices in a large number of Brdu-positive cells14days after spinalcord injury,21days after28days gradually reduced.④No significantdifference in BBB scores of two groups of rats at each time point, and nosignificant change over time.Conclusion: Methylprednisolone inhibit theproliferation and migration of endogenous neural stem cells after spinalcord injury. |
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