Study On Reduction Of The Residual Risk Of Transfusion-transmitted Diseases By NAT Detection In Volunteer Donors

Objective: To understand the blood of unpaid blood donors in Urumqi region byenzy-me linked immunosorbent assay (ELISA) for security and reliability, to explore thefeasibility of screening using nucleic acid amplification technology (NAT) for blood, tofurther improve the method in order to screening of blood donors to reducetransfusion-transmitted disease residual risk. Methods:(1) two ELISA reagents that fromDifferent kit manufacturers detect Hepatitis B Surface Antigen (HBsAg), hepatitis Cantibody (anti-HCV),human immunodeficiency virus antibody (anti-HIV) in blood ofunpaid blood donors；(2）NAT blood screening system detects HBV DNA, HCV RNA,HIV RNA of blood of every unpaid blood donors;(3) ELISA assay was negative, theNAT detection of positive samples for confirmation sent to the Shanghai KeHua company,and tracking and analysis of blood donors blood on a regular basis. observed with orwithout seroconversion to determine infection status. Results: were screened Urumqiregion unpaid blood donors March2011to October,14,696cases, of which a total of152cases positive by ELISA: HBsAg positive rate was0.52%(76/14696), anti-HCV positiverate was0.36%(53/14696), anti-HIV-positive rate was0.16%(23/14696); the NATdetection was positive in a total of71cases: HBV DNA positive rate of0.22%(32/14696),HCV RNA-positive rate of0.17%(25/14696), the HIV RNA-positive rate of0.10%(14/14696);14,544cases of ELISA-negative specimens was not detected by NATdetection of HCV RNA, HIV RNA-positive specimen, detected two cases of HBV DNApositive samples, after tracking analysis, the first case occurred seroconversion "windowperiod" infections, the second cases did not occurred seroconversion, but hepatitis B coreantibody was persistently positive,is occult hepatitis B. Conclusion: ELISA for detectionof blood safety has good protection, but remain residual risk of transfusion-transmitteddiseases,, NAT detection can reduce blood transfusion residual risk, improve the safety of blood transfusion.