The Original Generation Cervical Cancer Cell Culture, Identification Of Chemotherapy Drugs And Sensitive Experiment

Objective: Using cell culture technology to separate and culture cervical squamouscell in vitro, using MTT method to discuss the influence of paclitaxel, cisplatin,adriamycin and5-fluorouracil on the proliferation ofhuman cervical cancer primary cellcultured in vitro. Method: Using enzyme digestion method to separate and culturesquamous cell, Using inverted microscope to observe the morphology and proliferation ofthe cell, using immunofluorescent to identify the expression of cell surface makers CD44and CK17on squamous cell, using MTT to text the inhibition of paclitaxel, cisplatin,adriamycin and5-fluorouracil on the proliferation of the Primary cervical cancer cells.Result:1) cervical cancer cells isolated from in vitrohave the abilitiy of continuouspassage.The expression of CD44and CK17detected by immunohisto-chemical waspositive.2) MTT shows that paclitaxel, cisplatin, adriamycin and5-fluorouracil caninhibite the proliferation of primary cervical cancer cells, compared withcontrol group, theinbition effect of different time period group and different concentration grouphas asignificant difference. P＜0.05. Conclusion: cervical cancer cells can isolated、culturedand pass down in vitro; MTT shows that the four drugs using in the experimenthas theinhibition effect on the proliferation of primary cervical cancer cells, the sensitivity of4drugs in6specimens is different.In3of them, the sensitivity is: cisplatin＞paclitaxel＞adriamycin＞5-fluorouracil; in2of the6specimens, the sensitivityis paclitaxel＞cisplatin＞adriamycin＞5-fluorouracil; in of the specimens, thesensitivityisadriamycin＞cisplatin＞paclitaxel＞5-fluorouracil.The result indicates that, the sensitivity of samechemotherapeutic drug on different individuals is different, and the effect of inhibitiondepends on the time and concentration.