The Study Of Methylaiton Genes And Their Pathology Meaning In Kazakh Esophageal Squamous Cell Carcinoma

Objective: To investigate the methylated genes and their pathological significancein Kazakh esophageal squamous cell carcinoma (ESCC). Methods: The different genesbetween ESCC cells treated with5-aza-CdR for96h and control groups were screenedby human genome oligonucleotide microarrays with fluorescent dye swap method. Themethylated gene status and expression in both cells and tissue samples of ESCC wereverified by MSP and qRT-PCR, respectively. The relationship between selectedmethylated genes and the pathological parameters of Kazakh ESCC were analysed.Results: After treated with5-aza-CdR for96h, the morphology of ESCC cells displayedthat cells number were reduced. Microarray analysis showed that384genes weredifferentially expressed between ESCC cells treated and untreated groups with5-aza-CdR, including303up-regulated genes and81down-regulated genes. Amongup-regulated genes, the expression of TFPI2gene was raised11.42folds. Themethylation of TFPI2were obtained in untreated cells, and unmethylation of TFPI2wereobtained in5-aza-CdR treated cells. The methylation ratio of TFPI2was highly (45%) inKazakh ESCC compared with cancer adjacent tissue, the expression of TFPI2wasobviousely different in ESCC cell and Kazakh ESCC (p＜0.05), and the methylation ofTFPI2were significantly correlated with the differentiation grade of Kazakh ESCC（p＜0.05）. AKAP12gene expression were raised4.05folds, and the methylation of AKAP12was low in ESCC, meanwhile, there were no correlation between AKAP12and theclinical pathological parameters of Kazakh ESCC. Conclusion: Firstly,5-aza-CdR couldalter the abnormal methylation of certain genes in ESCC. Secondly, there was acorrelaiton between the methylation of TFPI2and Kazakh ESCC carcinogenesis and celldifferentiation. Thirdly, the methylation of AKAP12was low in ESCC.