Study On The Differentially Expressed Genes Between AIS And MIA By Genechip And Then Making A Preliminary Identification Of The Candidate Gene

ObjectiveAIS has a distinct histological pattern of tumor cells growing along a preexisting alveolar framework without evidence of stromal, pleural, or vascular invasion. However, Minimally invasive adenocarcinoma is defined as a tumor of less than3cm in diameter with an invasive part of less than5mm and Lepidic predominant Whereas AIS is uncomm on,MIA is a common tumor. MIA comprises approximately50%of all adenocarcinomas. Patients with AIS have much better chances of surviving than those with MIA. Some scholars have suggested that atypical adenomatous hyperplasia (AAH),AIS,MIA, and solid adenocarcinoma might represent the developmental sequence of bronchioloa lveolar stem cells rather than a classification system.Stepwise progression from AAH through AIS to invasive lung MIA was thus proposed.MethodsTotal RNA was extract from16lung tissue specimens.includig8AIS and8MIA. The AIS and MIA were analyzed for gene expression profiles using the Agiilent4*44K arrays. Gene Ontology analysis was used to define the genes which were considered candidate marker genes for tumor progression and metastasis. Diffe rentially expressed candidate genes were validated using quantitative real-time PCR. The SPSS software was used for the analysis.Two-sided values of P were given, and statistical signifcance was set at P<0.01.Results(1)In the basic of gene chips, there are279high expression genes and302low expression genes in the lung adenocarcinoma tissue.(2) MIA demonstrated increased expression of23genes and reduced expression of20genes compared with AIS. These genes were considered candidate marker genes for tumor progression and metastasis.(3) Of the43validated genes.CLDN18and ATF-2were found to have>10fold higher expression in AWBF,and the p-value was<0.01. CLDN18and ATF-2which represent invasion and tumor progressionConclusion(1) We found a new way how to screen signal transduction-regulate genes from gene chips in lung adenocarcinoma and then make a preliminary identification of the Candidate Gene.(2) CLDN18and ATF-2may be related with the progression of AIS to MIA. Furthermore, targeting of these genes may be a promising way for MIA treatment.