Study On The Quantity And Function Of Dendritic Cells In Immuno-related Pancytopenia

Objective:To study the quantity and function of Peripheral Blood (PB) DCs of the patients with IRP and explore the role of DCs in the pathogenesis of this disease.Methods:Thirty-seven untreated IRP patients, twenty-eight recovered IRP patients and seventeen healthy donors were enrolled in this study.The quantity of mDC (Lin-HLA-DR+CD11c+cells)、pDC (Lin-HLA-DR+CD123+cells) in PB and their expression of CD80、CD86were examined by FCM. The correlations between mDC, pDC, pDC/mDC,the expression of CD80, CD86on mDC, the expression of CD80, CD86on pDC and Thl/Th2, CD19+/BMMNC, the quantity of BMMNC-antibody (IgG)、the quantity of blood cells were analyzed.Results:1. The ratio of pDC in PBMNC was (0.91±0.64)%in untreated IRP, which was significantly higher than that of remitted patients (0.39±0.11)%and controls (0.29±0.13)%(p<0.01, p<0.01),while the ratio in remission group was higher than that of control group (p<0.05). The ratio of mDC in PBMNC was (0.21±0.20)%in untreated IRP and (0.34±0.21)%in remission IRP, There was no statistical difference between them and control group (0.29±0.09)%(p>0.05). The ratio of pDC to mDC in untreated group was6.75±6.11, which was significantly higher than that of remitted patients(1.55±0.93)and controls(1.07±0.43)(p<0.01, p<0.01), There was no statistical difference between the ratio of remission group and control group (p>0.05)2. The expression of CD80on pDC was (6.31±4.49)%in untreated group, which was significantly higher than that of remitted patients (3.09±2.93)%and controls (2.33±2.25)%(p<0.05,p<0.05), There were no statistical difference between the expression in remitted group and controls (p>0.05). The expression of CD86on pDC was(82.47±13.17)%in untreated group and(60.08±14.29)%in remission group, which were significantly higher than that of controls (47.95±18.59)%(p<0.05, p<0.05), while the expression in untreated group was higher than that of remission group (p<0.05). The expression of CD80on mDC was (6.20±5.44)%in untreated group and (3.97±3.24)%in remission group, which were significantly higher than that of controls (1.86±1.73)%(p<0.05, p<0.05), There were no statistical difference between the expression in untreated group and remission group (p>0.05). The expression of CD86on mDC was (97.06±4.82)%in untreated group and (95.58±6.51)%in remission group and (91.35±12.2)%in control group, while the expression in untreated group was higher than that of control group (p<0.05)3. The ratio of pDC in PBMNC was negatively correlated to Thl/Th2(r=-0.347, p<0.05), and positively correlated to the quantity of BMMNC-antibody(IgG)(r=0.606,p<0.05) and CD19+/BMMNC (r=0.709,p<0.05),while it was negatively correlated to the level of hemoglobin (r=-O.381,p<0.01) and platelet (r=-0.343, p<0.01). The ratio of mDC in PBMNC was positively correlated to Thl/Th2(r=0.595, p<0.05) and the level of hemoglobin (r=0.292, p<0.05).pDC/mDC was negatively correlated to Thl/Th2(r=-0.395,p<0.05), it was positively correlated to the quantity of BMMNC-antibody(IgG)(r=0.421,p<0.05) and CD19+/BMMNC (r=0.423, p<0.05),while it was negatively correlated to the level of hemoglobin and platelet (r=-0.304,p<0.05)(r=-0.287,p<0.05). The expression of CD80on pDC was negatively correlated to the level of hemoglobin and platelet (r=-0.431, p<0.05)(r=-0.464, p<0.05). The expression of CD86on pDC was negatively correlated to Th1/Th2(r=-0.733,p<0.05), it was positively correlated to the quantity of BMMNC-antibody(IgG)(r=0.283,p<0.05) and CD19+/BMMNC (r=0.436, p<0.05),while it was negatively correlated to the level of hemoglobin、platelet and white blood cell (r=-0.539,p<0.05)(r=-0.519,^<0.05)(r=-0.567,p<0.05).The expression of CD80was negatively correlated to the level of hemoglobin and white blood cell (r=-0.262, p<0.05)(r=-0.31, p<0.05),while the expression of CD86on mDC was negatively correlated to the level of white blood cell (r=-0.245, p<0.05)Conclusions:There exists increased quantity and hyperfunction of pDC in IRP patients, they may play important role in the pathogenesis of IRP. pDC may be regarded as potential new therapeutic targets of IRP.