Study On The Physico-chemical And Biocompatibihty Of Calcium Silicate-phosphate Bong Cement

Experimental purposes: In order to adjust the setting time and degradability of (tetracalciumphosphate TTCP+dalcium hydrogen phosphate DCPA) system bone cement, optimize itscompressive strength, improve its bioactivity, we try to compound C3S that we have prepared toTTCP+DCPA system bone cement, and explore the physical and chemical properties of thecomposite bone cement.Experimental methods:(1) The stting time of composite bone cement was determined by theGill Morocco (Gilmore) Determination of needle; the mechanical properties of bone cement wasestimated by the compressive strength of composites; after soking in the simulated body fluids(SBF), the changes of dissolution extracts pH value and the weight of bone cement along soakingtime, and combining the micro-morphology of the bone cement which was observated by SEMand XRD, described the dynamic change of bone cement in the human body.(2) Combining MTTexperiment and the real time PCR to research the biocompatibility of the compounded bonecement.Experimental results:(1) With the percentage of C3S increasing, the setting time of bonecement increased correspondingly.(2) The compressive strength of composite bone cement wasenhanced first, and then reduced along the percentage of C3S increasing. When the percentage ofC3S was40%, the compressive strength of cement reach its maximum21.6Mpa, which washigher than the strength of traditional (TTCP+DCPA)bone cement.(3) The composite bone cement has good degradability, and the degradability increased along the percentage of C3Sincreasing. When the percentage of C3S was40%, the degradability of cement reach itsmaximum10.26%. After immersion for7d, the compound bone cement maintained highercompressive strength, the maximum was27.84, and the pH value of soaking liquid closed toneutral.(4) Adding C3S bone cement can stimulate L929cell proliferation and improve theexpreesion of CyclinD1、PCNA、SDH gene.Experimental conclusion: The bone cement mixing with C3S prolonged the setting time of(TTCP+DCPA)system bone cement, improved its degradability and enhanced the compressivestrength a bit. This compound bone cement overcame shortcoming poor degradability of (TTCP+DCPA) cement, and has suitable setting time, higher compressive strength and excellentbiocompatibility and security, therefor, it might be taken as a potential bone cement repairalternative materials.