Preliminary Study Of Regulation Of β-catenin By MicroRNA In Cancer Cells

MicroRNA plays an important role in various diseases like cancers. In this study,the preliminary study of regulation of β-catenin by microRNA in cancer cells wascarried out. First at all, the cell model was established for the purpose of theexpression levels of β-catenin were affected by different concentration of aflatoxin.Six putative hsa-miRNA targeting β-catenin were obtained by bioinformatics analysis.Western blot was employed to examine whether these six miRNAs could regulateβ-catenin levels. hsa-miR-33a and hsa-miR-340appeared to downregulation theexpression of β-catenin. miR-33a negatively regulated the expression of β-cateninwith its mature form of hsa-miR-33a. The inhibition of the endogenous hsa-miR-33aaslo proved the effect of hsa-miR-33a on β-catenin. Cell viability, cell colony abilityand cell migration ability were study with overexpression of hsa-miR-33a, inhibitionof hsa-miR-33a, or transfection of siRNA of β-catenin. It revealed that hsa-miR-33acould downregulate the expression of β-catenin, and then inhibited cell viability,colony formation and cell migration.These results indicated that miR-33a suppressedcancer cell through its direct effect on β-catenin. Research would provided thereticalreference and well perspective for the application of miR-33a in the clinical therapyof cancers.