Relationship Between The Levels Of Cytokines (IFN-γ、IL-4) In Patients With Chronic Hepatitis B And Liver Injuries

Objective Hepatitis b virus (HBV) infection is in high incidence in the world,its pathogenesis is quite complex: hepatitis B virus itself does not have the pathogeniccells, either acute or chronic liver injury is by the host immune response against thevirus. The reaction against the liver cells exposed to the virus surface antigen orautoantigen expression, so clear the virus also caused liver injury.The cytokines are the general designation of low molecular protein factors withhigh activity and various functions, which are secreted by active immune cells andsome other cells. They are very important to regulate the immune system. Help T cellsare one kind of the main cells to secrete cytokines. They can be classified as Th1andTh2now. its representative cytokines are IFN-γ and IL-4. The balance of Th1/Th2isnecessary to maintain the normal immune functions of the body. The unbalance ofbody’s immune response to HBV is one of important pathogenesis of chronic hepatitisB. as the hypoimmunity of Th1and the hyperimmnunty of Th2.Currently the diagnosis of liver disease relies on clinical symptoms, signs,serology and imaging studies, but some patients after liver damage, there is no obviousclinical signs and serological and imaging indicators of change, which gives theclinical diagnosis and the treatment of choice and bring a lot of prognosis difficult.Liver biopsy can be clearly observed in liver and liver cell micro-changes, but the liverbiopsy has its limitations, for is not easy for the patients receiving.not universalcarried out. Through the liver biopsy of patients with chronic hepatitis B, to understandthe pathological diagnosis and clinical diagnosis of fibrosis, inflammation consistentrate,and intends to observe patients with chronic hepatitis B (different inflammation,fibrosis staging), whether the differences in serum levels of IFN-γ and IL-4couldsearch for a kind of non-invasive reflection of the more sensitive indicator of liver injury.Method: collected15cases of healthy peripheral blood from Second AffiliatedHospital of×××××l College and42cases of patients with chronic hepatitis B inliver centers in peripheral blood from×××××. As a control group of healthyvolunteers in15cases, male10cases,5females, aged28-59years.42patients withchronic hepatitis B, according to liver biopsy,four cases in which hepatitis B viruscarriers.in which chronic hepatitis B (CHB) group of19patients with mild, moderate10cases,13cases of severe group,34cases of male,8females. Aged18-59years. wedrew fasting wenous blood, added3ml venous blood into a tube, centrifugalized at4℃,1000r/min for15minutes, and then collected the serum specimen and storaged itin frige at-20℃.According to assay procedure in the introduction of enzyme-linkedimunosorbent assay kit, we dispensed100μl of standards and samples into theappropriation microtitre wells,20minutes before the test kit from the refrigerator out tobalance to room temperature,read the optical density at492nm with a mocrotiter platereader, and values based on standard hole standard curve, calculate the sample in theIL-4, IFN-γ levels, Test data as mean±standard deviation(X±SD), said analysis ofvariance, and other statistical methods for statistical analysis.Results1、IFN-γ、IL-4at the level of the three experimental groups: mild chronic hepatitisB group,the average level of IFN-γ、IL-4(47.83±19.79)、(98.00±43.97),moderatechronic hepatitis B group, the average level (60.20±22.19)、(91.80±24.80),severechronic hepatitis B group was (50.00±21.03)、(96.00±35.32), the level of all threegroups than the control group, the results were significantly different(p<0.05).(unit:pg/ml)2、each group IFN-γ/IL-4levels: control group mean (1.25±0.34), mild chronichepatitis B group (0.58±0.28), moderate chronic hepatitis B group, theaverage level (0.68±0.21), severe chronic hepatitis B group (0.62±0.18), thecontrol group was higher than the other three groups, the results aresignificantly different (p <0.05). 3、three different groups of chronic hepatitis B. IFN-γ/IL-4in comparison:combination of chronic hepatitis B, chronic hepatitis B with mild to severe chronichepatitis B group was less than moderate group, the results are significantly different(p <0.05); but mild chronic hepatitis B group and severe group was no significantdifference (p>0.05).4、Compare to healthy control group,6cases of normal transaminases patientsIFN-γ、IL-4significantly increased.Conclusion1、Imbalance of Th1、Th2cytokine expression, reduced in Th1cells and its immuneresponse, increased in Th2cells. The body’s cellular dysfunction is one of the reasonscausing persistent infection of HBV.2、The levels of Th1、Th2cytokine does not increase by the change of pathologywith chronic hepatitis B.3、The increased expression of Th1、Th2type cytokines can be used as an indicater...