Effects And Mechanisms Of Nimesulide On Proliferation In Human Hepatocellular Carcinoma HepG2Cells Under Hypoxia Environment

Objective：To investigate the effects and mechanisms of Nimesulide on proliferation inhuman hepatocellular carcinoma HepG2cells under the hypoxia environment.Contents and methods of research：HepG2cells were divided into6groups:normaxia(21%O2)+cells with notreatment,nomaxia+cells with50μmol/L Nimesulide,hypoxia(1%O2)+cells with notreatment, hypoxia+cells with50μmol/L Nimesulide, hypoxia+cells with100μmol/LNimesulide, hypoxia+cells with200μmol/L Nimesulide.The cell proliferation wasexamined by methyl thiazolyl tetrazolium(MTT) assay.The expression levels ofcyclooxygenase-2(COX-2),HIF-1α and survivin mRNA were detected byRT-PCR.The COX-2,HIF-1α and survivin protein expression were measured byWestern blotting.Results:Nimesulide inhibited the proliferation of the HepG2under the hypoxiaenvironment.Compared with cells under nomarxia environment,the expression ofCOX-2,HIF-1α and survivin of the cells under hypoxia environment werehigher(P<0.05).Compared with the hypoxia(1%O2)+cells with no treatmentgroup,the expression levels of COX-2and survivin mRNA of HepG2were obviouslower in the treatment group(P<0.05),the expression levels of COX-2,HIF-1α andsurvivin protein were significantly decreased(P<0.05).Conclusion:Nimesulide can inhibit proliferation on HepG2cells.The possible mechanismsare given below, Nimesulide can inhibit the activity of COX-2or reduce theexpression of HIF-1α protein.