| Objective: To investigate the effect of restraint stress and diabetes onperiodontitis model in rats.Method:96SD rats′maxillary first molor on the right received stainlesssteel wire ligation to be induced experimental periodontitis. Then theywere divided into four groups randomly: diabetic periodontitis associatedwith restraint stress for12hours every day（G1）, periodontitis associatedwith restraint stress for12hours every day（G2）, periodontitis associatedwith diabetes（G3）, periodontitis（G4）,and the non-ligated left first molorin maxillay in G4is considered to be the blank controlgroup(G5).Diabetes were induced by intraperitoneal injection ofstreptozocin(STZ).6rats were sacrificed by decapitation in each groupafter2,3,4,5weeks. Marginal tissues around ligated teeth were received,and inflammatory factors were analysed by immunohistochemistrymethods. The pathology of periodontal tissues was obtained by HEstaining. Alveolar bone loss(ABL), sulcus bleeding index (SBI)andprobing depth (PD) were measured.Results:(1) After2,3,4,5weeks, ABL,SBI,PD in G3were significantly different compared to other groups (P<0.05), and G3>G1>G2>G4>G5.(2)The pathology of periodontal tissues showed that the alveolar bone crestdescended significantly in G3, and a significant loss of connective tissueattachment and bone loss in the experimental teeth.(3)IL-1β and TNF-αwere significantly observed in G3.(4) The blood glucose in G1wassignificantly higer than G3.Conclusion:(1)Stress can promote the inflammation of periodontal tissuein rats,and stress is a risk factor of periodontitis.(2)Stress can rise theblood glucose.(3)Stress assotiated with diabetes can significantly increasethe inflammation of periodontal tissue in rats. |
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