Experimental Research On Treatment Of Chronic Heart Failure Rat By Ultrasound Microbubble-Mediated Heart Fatty Acid Binding Protein Gene

Objective To determine the effect of heart fatty acid binding protein(H-FABP) gene transfected by ultrasound targeted microbubble destruction (UTMD) technology on cardiac function after post-infarction.Methods Myocardial infarction (MI) was induced in wistar male rat (n=46,220±20g) by ligation of the left anterior descending coronary artery. After8weeks, the31surviving rats with heart failure were randomly divided into4groups:①H-FABP+ultrasound+microbubble group (H-FABP+US/MB group,n=8), ultrasound-targeted destruction microbubble loaded with H-FABP gene was transfected to rats heart;②ultrasound+microbubble group (US/MB group,n=8), ultrasonic destruction of microbubbles loaded without gene;③H-FABP+normal saline group (H-FABP+NS group,n=8), normal saline containing gene were infused from jugular vein;④surgery alone group (SA group,n=7), only normal saline were infused from jugular vein;⑤sham surgery group (SS group,n=6).After transfected14days, the cardiac function of all the rats were measured, H-FABP expression in non-infarct region of left ventricular was detected by Western blot,free fatty acid (FFA) content of venous blood and non-infarct region myocardium were detected by ELISA,lipid peroxidation in non-infarct region of left ventricular was detected by thiobarbituric acid reactive substances (TBARS) method,the iNOS expression in non-infarct region of left ventricular was detected by immunohistochemical assay.Results Compared wtih sham surgery group, left ventricular ejection fraction(LVEF) and the H-FABP expression were decreased significantly (P<0.05), free fatty acid content and malondialdehyde (MDA) level and the iNOS expression were obviously increased (P<0.05) in the MI groups.Compared with US/MB,H-FABP+NS,SA groups, LVEF and the H-FABP expression were apparently increased (P<0.05), free fatty acid content of myocardium and MDA level were obviously decreased (P<0.05) and the iNOS expression was markedly inhibited (P<0.05) in H-FABP+US/MB group.Conclusion Ultrasound microbubble-mediated H-FABP gene transfection improves cardiac function via upregulating H-FABP,reducing of myocardial FFA,ameliorating myocardial oxidative stress and inhibiting iNOS expression.