Effects Of5-aza-2dc On SLIT2Gene Methylation Status And Malignant Phenotype On Hepatoma HepG2Cells

Objactive To evaluate5-aza-2dc’s effect on SLIT2gene methylation status and cellgrowth, migration, invasion and apoptosis on hepatoma cell line HepG2, and to explore thepossible molecular mechanism. Methods HepG2cells were treated with differentconcentration of5-aza-2dc respectively. The change of SLIT2gene methylation status inhuman hepatoma HepG2cells was analyzied by Methylation-speciafic PCR. Theproliferation of HepG2cells was observed by MTT assay with different concentration andtime of5-aza-2dc. And then, the HepG2cells were treated with5-aza-2dc at the dose of10μmol/L as a treated group. At the same time set the cell control group and DMSO controlgroup. Wound healing test and invasion assay was applied to measure the change ofmigration and invasive ability respectively. Meanwhile, the cell apoptosis rate was detectedby Annexin V-FITC/PI assay. Results After treated with5-aza-2dc, the methylation statusof SLIT2gene had been reversed to some extent and showed a dose-dependent; After48hin wound healing assay, the control group of HCC cells moved to the center of the scratch,and drug treatment group was inhibited; After24h in cell migration assay, drug treatmentgroup of HCC cells’ invasive ability was inhibited comparing with control group (p<0.05);In Annexin V-FITC/PI assay, the cell apoptosis rates of the drug treatment group showed asignificant increase comparing with control group (p<0.01). Conclusion5-aza-2dc caninhibit the proliferation, promote the apoptosis and inhibit the migration and invasion ofHepG2cells in vitro, moreover, the inhibition of cell migration and invasion may be accomplished by reversed the methylation and restored the expression of SLIT2gene.