Association Between C-X-C Motif Chemokine10(CXCL10) Polymorphism And Susceptibility To Hepatitis B Virus Infection

Background Chronic hepatitis B virus (HBV) infection was reguarded as a multi factors and polygenic disease, which was resulted in the complex interaction among virus, host, and environmental factors. Many studies have documented that the genetic factors may play an important role in the process of HBV infection. To absereve the susceptibility gene may be a critical juncture to solve the persistent infection with the HBV. Genetic susceptibility to Infection disease may decide many founctional subareas, including antigen presenting, receptor recognition and the recruition of effector lymphocytes. C-X-C Motif Chemokine10(CXCL10) recruit lymphocytes from peripheralblood by interacting with their receptors, which mediate the viral clearance and immunologicmjury in chronic hepatitis B infection. So the single nucleotide polymorphism (SNP) of CXCL10gene may be associated with HBV infection and vary clinical outcomes.Objective To investigate the association between two SNPs in CXCL10and the genetic susceptibility to chronic HBV infection.Methods We performed a case-control study of501chronic HBV infections as cases and301acute self-limiting HBV infected individuals as controls. SNPs in CXCL10(rs4257674, rs4256246) were genotyped with the Multiplex Snapshot technique. The genotype, allele and haplotype frequencies were calculated and analyzed between the two samples. Result1. The genotype frequencies for CC, CT and TT of rs4257674of CXCL10gene were83.6%,15.0%,1.4%in501case samples, and92.4%,7.3%,0.3%in control samples. Duing to little TT genotype, genotype CT and TT were combined to compare with CC. the genotype distribution in the controls was significantly different from that of the patients (P=0.0002). Compared with genotype CC, the risk for the individual who carry genotype CT, TT had an increased susceptibility to develop into chronic HBV infection was2.364times (OR=2.3641,95%CI:1.4535-3.8462, P=0.0002).2. The allele frequencies for C, T of rs4257674of CXCL10gene were91.1%,8.9%in501case samples, and96.0%,4.0%in control samples. There was a significantly difference between the two samples (P=0.0002). Compared with genotype CC, the risk for the individual who carry genotype CT, TT had an increased susceptibility to develop into chronic HBV infection was2.3474times (OR=2.3474,95%CI:1.4780-3.7286,P=0.0002).3. The genotype frequencies for CC, CT and TT of rs4256246of CXCL10gene were41.9%,12.0%,46.1%in501case samples, and40.5%,15.3%,44.2%, in control samples. No statistically significant difference between cases and controls (P=0.4076).4. The allele frequencies for C, T of rs4256246of CXCL10gene were91.1%,8.9%in501case samples, and96.0%,4.0%in control samples. No statistically significant difference between cases and controls (OR=1.1069,95%CI:0.8972-1.3656, P=0.3413).5. C-C, C-T, T-C and T-T haplotypes frequency, generated by the two SNPs, were56.9%,8.1%,34.2%,0.8%in the case samples,58.6%,4.0%,37.4%,0%in control samples. C-T haplotype showed a significant difference between the two groups (OR=2.139,95%CI:1.340-3.413, P=0.0011). The risk for the individual who carry C-T haplotypes had an increased susceptibility to develop into chronic hepatitis B was2.139times. Despite, C-C haplotypes (OR=0.948,95%CI:0.772-1.164, P=0.6090), and T-C haplotypes(OR=0.883,95%CI:0.715-1.090, P=0.2468) had no statistically significant differences between the two samples. T-T haplotype frequency less than0.03were ignored in analysis.Conclusion1. Our results suggest that the SNP site rs4257674of CXCL10may be associated with HBV persistence infection, TT was a susceptible genotype for HBV persistence infection, The patients who carry C allele have a higher probability to develop into chronic HBV infection than that who carry T allele, therefore, we conclude that C allele may be a protective gene, but T allele may be a susceptible gene in the Han Chinese population.2. The SNP site rs4256246of CXCL10may be not associated with HBV persistence infection in the Han Chinese population.3. C-C haplotype, generated by the rs4257674and rs4256246may be associated with HBV persistence infection in the Han Chinese population.