Preparation And In Vitro Biocompatibility Evaluation Of The Nano-titanium Calcium Coating Of Titanium Alloy

Objective:This study was aim at comparing the cytocompatibility of titanium substrate, HA coating and novel CaTiO3coating material to osteoblasts, providing a preliminary evaluation of its potential to future application as new substitution of implants.Methods:Osteoblasts MC3T3-E1was employed to culture on three groups of materials (group A:titanium without coating; group B:HA coated titanium substrate; group C:CaTiO3coated titanium substrate). Calcein-AM+PI+DAPI fluorescent staining and MTT method was employed to evaluate the adhesion and proliferation of osteoblasts. Actin, β-tubulin and DAPI immunofluorescent staining was employed to investigate maturation of osteoblasts. We also measured the collage I expression and ALP activity to detect the osteogenic activity of materials. SEM was employed to observe calcium nodule formation directly.Results:Higher cell density, MTT valve, collage I expression and ALP activity was detected on group B and C, comparing to group A (P<0.05), and no significant difference existed between group B and C (P>0.05). Cytoskeleton immunofluorescent staining reveals widely stretched osteoblasts and well developed pseudopods only on group B and C materials. SEM observation revealed obvious more and larger calcium nodule formation on group B and C materials. Conclusion:Our novel CaTiO3coating material has high osteogenic activity and low cytotoxicity to osteoblasts. This study demonstrated the similar biocompatibility of CaTiO3coating with the HA material, implied its potential application to future biomaterial in orthopedics.