ATRA Regulates Cell Progression Through The GPI-PLD

Objective:To investigate the expression of glycosylphosphatidylinositol specific phospholipase D (GPI-PLD) in leukemia cell HL-60after ATRA added to the culture medium, to find out the relative function of GPI-PLD in the course of leukemias generating and developing, as well as to make out the influence of ATRA on GPI-PLD.Methods:Using RT-PCR to detect the expression level of GPI-PLD mRNA in HL-60cell line after ATRA added to the culture medium. GPI-PLD activities were analyzed quantitatively by TX-114partition with GPI anchored placental alkaline phosphatase (PLAP) as a substrate. The GPI anchored CEA and CD87detected by ELISA. MTT was used to detect the proliferation in each groups, and the cell circle of leukemia cell induced by ATRA with the flow cytometer.Results:After ATRA added to the culture medium, the mRNA expression and activities of GPI-PLD in HL-60cell line greatly increased. The level of GPI anchored CEA and CD87releasing was increased. The proliferative capacity was obviously decreased and the cell cycle was arrested in G2/M phase.Conclusions:After inducer ATRA added to the culture HL-60cells, the activity and expression of GPI-PLD are all increased, the releasing of GPI anchored proteins is also increased by GPI-PLD. The study shows that the expression of GPI-PLD probably has contact with leukemias generating and developing.