Study On The Protective Effect And Related Mechanism Of Troxerutin On Rat Acute Liver Failure Induced By D-GalN/LPS

Object:To explore the possible mechanism of rat acute liver failure model induced by D-GalN/LPS.To observe the protective effect of troxerutin in the acute liver failure of rat induced by D-GalN and LPS and explore the possible mechanism.Methods:①D-GalN400mg/kg and LPS0.5mg/kg was injected into abdominal cavity which administrated the acute liver failure of rat.Troexrutin150mg/kg body weight was lavaged as therapy.②56male rats were divided into three groups randomly:normal group(n=8),control group(n=24).therapy group(n=24).③The control group and therapy group were given D-GalN/LPS injection.While the normal group were injected saline.The therapy group was lavaged by troxerutin,1time/day for3days before establishing the model.The normal group and the control group were lavaged saline.To inject the D-GalN/LPS2hours after been lavaged.The normal group were killed6hours after molding.The control group and the therapy group were killed at the time of6h、12h、18h after molding.④Compare the serum levels of ALT、AST and TB,the histopathological changes of the liver. Immunohistochemical to test the expression of NF-κB.RT-PCR to test the expression of TNF-α mRNA、 IL-6mRN A.Kit to test the activity of NO and MDA.Results:1. We succeeded in administering the acute liver failure model by intraperitoneal injection of D-GalN400mg/kg combined with LPS0.5mg/kg.The serum level of ALT、AST、TB in control group were significantly increased P<0.05.Massive necrosis and lots of inflammatory of hepatocellular parenchyma were observed histologically.The levels of NF-κB、TNF-α mRNA、IL-6mRNA and the activity of NO、MDA were significantly higher than the normol group.2. Compare the control group to therapy group:①The serum level of ALT、AST、TB in therapy group of three time points were significantly lower than the control group compared with control group at the same time,P<0.05.②The degree of liver injury in therapy group was significantly reduced than the control group.③The levels of NF-κB、TNF-α mRNA、IL-6mRNA and the activity of NO、MDA in therapy group of three time points were significantly lower than control group at the same time,P<0.05.Conclusion:Succeeded in administering the acute liver model of rats by intraperitoneal injection of D-GalN400mg/kg combined with LPS0.5mg/kg.The possible mechanism was that D-GalN/LPS increased the expression of TNF-α, IL-6, NF-κB, enhanced the activity of NO, MDA. Troxerutin plays an effectively protective role in the acute liver failure of rats,troxerutin can inhibit the inflammation of liver,decrease the necrosis.The possible mechanism was that troxerutin reduced the expression of TNF-α、IL-6、NF-κB,receded the activity of NO、MDA...