The Study Of Functionalized Collagen Membrane By Drug-loaded PLGA Nano-particles For Bone Regeneration

Object:The goal of this study was to develop a functional collagen membrane that was treated with poly (lactic-co-glycolic acid)(PLGA) nanoparticles loaded with dexamethasone (DEX) as a bioactive molecules for guided bone regeneration (GBR) in Vivo study.Materials and Method:In this study, ionic immobilization of DEX-loaded PLGA nanoparticle was performed on the collagen membrane surfaces. DEX-loaded PLGA nanoparticles were prepared using water-in-oil standard emulsion method. The produced nanoparticles were pre-coated with positively charged poly(ethyleneimmine)(PEI) molecules and then immobilized on the surface of collagen membrane, which were physically immobilized using counter charges of positively charged PLGA nanoparticles and negatively charged collagen membrane surface. Release profile of DEX over a4weeks immersion study indicated an initial burst release followed by a sustained release. The performance of this system was investigated using calvarial bone defect (8mm diameter) model of rats. In vivo evaluation of the defects filled with membrane containing DEX-loaded PLGA nanoparticle indicated enhanced volume and quality of new bone formation when compared to defects that were either unfilled or filled with membrane alone.Result:This innovative platform for bioactive molecule delivery more potently induced osteogenesis in vivo, which may be exploited in implantable membranes for stem cell therapy or improved in vivo performance.Conclusion:It was thus concluded that this newly developed collagen membrane treated with drug-loaded PLGA nano-particle might be applicable as a promising bone graft substitute for GBR.