The Study Of Effects Of Tashinone â…¡A-sulfoacid-natrum Injection On The Electrical Activity Divorce Between Endocardium And Epicardium Induced By Myocardial Ischemia And Lidocaine In Isolated Rabbit Hearts

Objective:To establish the model of the electrical activity divorce between endocardium and epicardium (EAD) induced by myocardial ischemia and lidocaine; To study the effect of Tashinone ⅡA-sulfoacid-natrum injection (TSN) on the EAD. Methods:1.The electrocardiogram of standard II leader (ECG) and surface electrocardiogram of epicardium (SECG1) and surface electrocardiogram of endocardium (SECG2) were recorded simultaneously in isolated rabbit hearts perfused by Langendorff apparatus;2. To establish the model of the EAD with pituitrin hypophysin and adrenaline and lidocaine;3. To observe the effects on the EAD treated by TSN. Fifty isolated hearts of New Zealand rabbits were randomly divided into five groups (n=10in each group),①Blank control group: the hearts were perfused for40minutes continuously;②TSN negative control group:TSN15mg was injected to the perfusate for40minutes;③Model group: The lidocaine60mg were administered after pituitrin hypophysin20u and adrenaline2mg in perfusate;④TSN pretreatment group:The same treatment with model group was given after TSN15mg for30min;⑤TSN treatment group: TSN15mg was administrated after the model was established. ECG and SECG1and SECG2were observed simultaneously.4. Statistics analysis:All the experimental datum were expressed as the mean value±standard difference (x±s), and dealt with by software SPSS13.0, the mean values comparision used one-way ANOVA, the difference was considered significance when the p value was less than0.05. Results:1. The changes in perfusion flow in unit time Compare blank control group (14.45±1.44ml/min) with TSN negative control group (13.62±1.29ml/min), there was no significant difference(P>0.05, similarly hereinafter); There was a significant difference between model group(8.22±0.86ml/min) and blank control group or TSN negative control group(P<0.05, similarly hereinafter); There was a significant difference between TSN pretreatment group(9.65±1.31ml/min) and blank control group or TSN negative control group; Compared with blank control group or TSN negative control group and TSN treatment group (7.85±0.64ml/min), there was a significant difference; There was a significant difference between model group and TSN pretreatment group; There was no significant difference between model.group and TSN treatment group; There was a significant difference between TSN pretreatment group and TSN treatment group.2. The changes in heart rate Compare blank control group (122±10bpm) with TSN negative control group(116±9bpm), there was no significant difference; There was a significant difference between model group(66±10bpm) and blank control group or TSN negative control group; There was a significant difference between TSN pretreatment group (82±5bpm) and blank control group or TSN negative control group; Compared with blank control group and TSN negative control group and TSN treatment group(69±13bpm), there was a significant difference; There was a significant difference between model group and TSN pretreatment group; There was no significant difference between model group and TSN treatment group; There was a significant difference between TSN pretreatment group and TSN treatment group.3. The changes in conduction time from endocardium and epicardium Compare blank control group(15.16±2.93ms) with TSN negative control group(15.48±2.73ms), there was no significant difference; There was a significant difference between model group(73.74±38.48ms) and other four groups; There was no significant difference between TSN pretreatment group(18.11±3.92ms) and blank control group or TSN negative control group; Compared with blank control group or TSN negative control group and TSN treatment group(33.88±11.29ms), there was a significant difference; There was a significant difference between TSN pretreatment group and TSN treatment group.4. The changes in ventricular arrhythmias. There was no arrhythmia in blank control group and TSN negative control group. In model group, TSN pretreatment group and TSN treatment group, there were several kinds of arrhythmias, including premature ventricular beat, ventricular tachycardia, intra-ventricular block, ventricular flutter, ventricular fibrillation and EAD. All arrhythmias mentioned above were occurred in model group and TSN treatment group, while only one EAD and no ventricular flutter and ventricular fibrillation were occurred in TSN pretreatment group. The duration of arrhythmia in model group and TSN treatment group and TSN pretreatment group were1835.4±469.51s、795.8±185.14s and448.4±109.18s respectively, there was a significant difference among three groups.5.The incidence rate and duration and transformation of EAD The EAD showed different frequency and rhythm between endocardium and epicardium, and there was no relation between each other. No EAD occurred in blank control group and TSN negative control group. The incidence rate of EAD in model group and TSN treatment group were both50%respectively, and10%in TSN pretreatment group. ECG is synchronous with SECG1in rhythm and frequency, however, the rhythm and frequency between endocardium and epicardium were separated during EAD. The duration of EAD in model group and TSN treatment group were1189±460.77s and368±266.88s, there was a significant difference between two groups. The transformation of EAD is different among the three groups. The four hearts stopped beating after EAD of five hearts in model group, and the other turned to multiple premature ventricular beat. In TSN pretreatment group, the EAD turned into ventricular rhythm. In TSN treatment group, no hearts stopped beating, and the EAD were transformed into ventricular rhythm.Conclusions:1. On the background of general myocardial ischemia and effect of adrenalin, lidocaine could induce electrical activity divorce between endocardium and epicardium.2. The rhythm and frequency in electrocardiogram was same as surface electrocardiogram of epicardium, however, the endocardium and epicardium were separated during electrical activity divorce.3. The electrical activity divorce between endocardium and epicardium tended to transform into ventricular arrest.4.Tashinone II A-sulfoacid-natrum injection could enhance the electrical stability of myocardium by improving myocardial ischemia, accelerating the conduction between endocardium and epicardium, thus reducing the occurrence and development of electrical activity divorce between endocardium and epicardium.