Effect Of Fasudil On The Expression Of RhoA/Rho-kinase In The Vaginal Smooth Muscle Cells Of Castrated Rats

AbstractObjective:RhoA/Rhokinase (Rock) is important in cell mitosisadhesion, adjustment of the cytoskeleton, muscle cell contraction, infiltration of tumor cells and et al. There are two types of Rock, Rock1and Rock2. Rock1and Rock2are serine/threonine protein kinase. Rock contains about1300amino acids, and the molecular mass is about160kD. Rock1and Rock2mainly distributed in the cytoplasm, and they adjust the cytoskeleton components. This study investigated the activity of Rock1and Rock2in the vaginal smooth muscle in castrated rats after the fasudil treatment, and the relationship between Rock and estradiol, and explore the effects of fasudil on Rock in the vaginal smooth muscle in castrated rats.Methods:30female Sprage-Dawley rats of10weeks were randomlydivided into6groups: groups A(4-week control group), B (6-weekcontrol group), C(4-week bilateral ovariectomy), D(6-week bilateralovariectomy), E(4-week bilateral ovariectomy plus treatment with fasudil)and F(6-week bilateral ovariectomy plus treatment with fasudil). Eachgroup has5rats. The group C、D、E and F remove the bilateral ovaries atthe same time. One week after operation, the group C and group D wereinjected with normal saline0.2ml/kg.day, while the group E and groupF were injected with fasudil10mg/kg.day.4and6weeks after the therapy of fasudil, the levels of serum estradiol were determined and theexpressions of Rock1and Rock2of each group were measured byWestern blot respectively. The experimental data to±s. Using theSPSS15.0statistical analysis software, estradiol level and Rock1andRock2band intensity ratios using single factor analysis of variance.Results: The levels of serum estradiol were significantly lower in groupC[(24.142±0.885) pg/ml] and group E[(23.584±1.526) pg/ml] thanin group A [(44.090±2.182) pg/ml](P <0.01). The levels of serumestradiol were significant lower in group D [(21.772±1.170) pg/ml] andgroup F [(20.946±1.136) pg/ml] than group B[(41.450±1.393) pg/ml](P <0.01). The levels of serum estradiol were no significantlydifferences in group C, group D, group E and group F(p＞0.05). Theexpressions of Rock1and Rock2were significantly more higher in groupC than group A and group E(P <0.05). The expressions of Rock1andRock2were significantly more higher in group D than in group B andgroup F(P <0.05). The expressions of Rock1and Rock2were nosignificantly differences in group A, group B, group E and group F(p＞0.05). Conclusion: The cytoplasm of vaginal smooth muscle cell inSprage-Dawley rat with RhoA/Rho kinase expression; The serumestradiol levels decreased significantly and vaginal smooth muscle Rock1and Rock2increased significantly after ovariectomy; Fasudil may improve the colpismus by decreasing the vaginal smooth muscletension through inhibiting the high expression of Rock1and Rock2.