Based Metabolomics In Hot And Humid With Chronic Hepatitis B In The Material Basis Of Retention Syndrome

Objective:The use of metabolomics methods,The comparative analys-is of serum metabolites in normal health group and GanDuQing particles before the treatment of chronic hepatitis B after damp heat resistance syndrome type group spectrum. From the molecular level to explore the material basis for the development of resistance in chronic hepatitis B damp heat syndrome type and GanDuQing particle retention syndrome type mechanism in the treatment of chronic hepatitis B hot and humid, Modern scientific basis for clinical diagnosis and treatment of chronic hepatitis B damp heat resistance card.Methods:Select outpatient physical examination results for hepatitis B infection history of20cases of healthy volunteers as controls,To do any treatment, compiled for the Ctrl;Select clinical meet the diagnostic criteria of chronic hepatitis B and underwent rigorous training by two associate professors of Chinese medicine practitioners dialectical damp heat resistance syndrome20cases;GanDuQing particles before treatment,20cases in the observation group, compiled for the TM1;15cases after GanDuQing particle therapy as a treatment group, compiled for the TM2, Record the various groups of specimens of liver function, HBV-DNA, B ultrasonic laboratory tests,Collected in the control group to observe the plasma samples100μL,Treatment group to use GanDuQing particle therapy (oral,3times a day, a10g, three weeks of continuous treatment).1week to detect a liver function, And records.Collection of plasma samples before and after treatment100μL. LC-MS technology for serum metabolite spectral analysis by liquid chromatography mass spectrometry;Partial least square discrimination analysis, and data processing to obtain the differences in plasma metabolites.Results:(1) In the PLS-DA analysis,Observation group, the treatment group and control group there is a clear separation of metabolic profiles;Treatment group than in the observation group significantly closer to the control group.(2) Observed compared with the control group,Plasma phosphatidylethanolamine、Phytosphingosine、9,10-DiHO DE4E,15Z-Bilirubin Ixa、Symmetric dimethylarginine、11’-Carb oxy-alp hatocotrienol、Lyso-PA、Lyso-PC(18:3)、Cholic acid glucuronide、 Glycochenodeoxycholic acid3-glucuronide、Taurochenodesoxycholic acid, Peak of the11compounds increased more obvious; phosphatidylch-oline and Lyso-PC(18:2)、two kinds of compounds, the peak decreased.(3) Compared with before treatment, the treatment group after treatment,Plasma Phytosphingosine, phosphatidylcholine, phosphatidylgl-ycerol, Mesobilirubinogen of Epsilon-(gamma-Glutamyl)-lysine or Glycochenodeoxycholic acid,3-glucuronide Glycochenodeoxycholic acid,3-glucuronide, The peak of the seven kinds of compounds were significantly lower than before treatment;Phenylalanylphenylalanine elevatedConclusion:①Metabolomics analysis results reveal that:Chronic hepatitis B damp heat resistance syndrome and health differences of the main ingredients of the metabolites.②the GanDuQing particles in the treatment of chronic hepatitis B hot and humid in the resistance-type effect is remarkable.③the GanDuQing particles treatment of chronic hepatitis B hot and humid in the resistance card to gain the better results may be related to adjustment related to the metabolism of lipids and amino acids.④The bilirubin, and dimethyl arginine, Gly goose deoxycholic acid, cholic acid, glucuronic acid, taurine, lysophosphatidic acid (LPA (18:2)) resistance in chronic hepatitis B hot and humidpotential markers of the permit.