The Study Of Relationship Between Intestinal Flora And Ulcerative Colitis

Objective: Through the qualitative and quantitative study of the faecal microfloratypes and specific bacterium with Ulcerative Colitis(UC) patients and normol group,respectively, to discovery the differences of intestinal microflora structure and changes ofnumbers in two groups in order to explore the relationship between intestinal microfloraand UC. Methods: Collected the fresh fecal samples of UC patients(n=35) and the healthypeople(n=35), respectively, sex, age and nationality were matched between two groups inaccordence with1:1proportion, then the bacterial genome DNA extracted from the humanintestine using kit. The universal primer for V3region was designed by a set of16SrDNA-targeted, the different structure of two groups’ intestinal flora were filtered outby DGGE-PCR, gel extraction, purification and clone of them was done before genesequencing, the categories were confirmed after the sequencing results were blasted inGenBank database. Group or species-specific primers for Escherichia coli, Lactobacillusand Bifidobacteria group were designed by a set of16SrDNA-targeted sequences to detecttheir numbers by RT-PCR quantitatively. Results: Lots of different strips existed inbetween UC group and normal group, the frequency of one strip of them had statisticallysignificant difference(P﹤0.05), which was the uncultured and not be classified bacteriumin GenBank database; the amounts of Escherichia coli, Lactobacillus group andBifidobacteria group were respectively(4.62±1.10;5.27±1.02),(4.99±0.75;4.65±0.95),(5.07±0.95;4.93±0.99) in the fecal samples between the normal group and UC groupafter RT-PCR. The numbers of Escherichia coli increased apparently in UC group(t=2.540,P=0.013), while Lactobacillus and Bifidobacteria group had no difference obviously(t1=0.488, P1=0.530; t2=-0.533, P2=0.596). Conclusion: The changes of intestinal floramight be related to the incidence of UC, which remains the further research of fullgenomic sequencing in intestinal microbiota.