Antineutrophil Cytoplasmic Antibodies Associated Small Vessal Vasculitis Patients Serum Inflammatory Factor And The Expression Of Visfatin

Objective:(1)Discussion on the value of Antineutrophil cytoplasmic antibodies(ANCA) and its target antigens-Proteinase3(PR3) and myeloperoxidase(MPO) in Antineutrophil cytoplasmic antibody-associated systemic vasculitis(AASV);(2) Observation of AASV patients with small vascular inflammatory activity period, serum inflammatory factor C-reactive protein(CRP), interleukin-6(IL-6), tumor necrosis factor alpha(TNF-a)and visfatin concentration changes and remission period to;(3) Analysis of major visceral fat secretion by Visfatin in AASV inflammatory injury and immune regulation function, and CRP, IL-6, TNF-a potential association between.Methods:In52cases of active AASV and25cases of remission in serum samples using the indirect immunofluorescence assay for detection of ANCA, Based on the fluorescence microscope and immunofluorescence staining of parts is divided into different cytoplasmic ANCA (cANCA) and nuclear cycle type ANCA (pANCA); Application of ELISA measurements of PR3, MPO, IL-6, TNF-a and visfatin; Immune turbidimetric method for the determination of the content of CRP.Results:(1) cANCA and pANCA of the respective identification corresponding to target antigens PR3and MPO, with a target antigen specificity (P<0.001), and a long time with the outcome of a disease and change (P>0.05).(2) Active AASV CRP, IL-6, TNF-a, visfatin levels were elevated compared with the normal control group(P<0.001); Activity phase and remission phase comparison of CRP (P<0.05), IL-6(P<0.001) content decreased to some degree; TNF-a did not significantly decrease (P>0.05); the content of visfatin and AASV in remission period continue to rise than before treatment (P<0.05). Remission period compared with the control group CRP (P<0.001), TNF-α(P<0.05), visfatin (P<0.001) still have difference, But IL-6was close to the normal control group (P>0.05).(3) The inflammatory factors in patients with AASV is in positive correlation, The CRP and IL-6correlation coefficient was0.471(P<0.001), CRP and TNF-a correlation coefficient was0.315(P<0.05), CRP and visfatin correlation coefficient was0.364(P<0.01), IL-6and TNF-a correlation coefficient was0.458(P<0.01), IL-6and visfatin correlation coefficient was0.577(P<0.001), TNF-a and visfatin correlation coefficient was0.550(P<0.001).Conclusion:ANCA and its target antigen PR3and MPO detection has an important diagnostic value, but because of its in vivo concentrations maintained for a long time, not by ANCA and its target antigen levels to predict vasculitis activity, treatment effects, relief and/or has recurred. Data display, AASV activity period compared with the control group, serum inflammatory factor CRP, IL-6, TNF-a and visfatin concentration was significantly increased, and the inflammatory factor and visfatin positive relationship between regulation relationship. In the remission phase of CRP, IL-6, varying degrees of downward, and TNF-a level not drop significantly, remission of serum visfatin content than the activity period is increased, suggesting that visfatin and TNF-a reciprocal regulation between the dose dependence of visfatin, this change may also herald AASV this chronic wasting disease in maintaining remission of visceral fat cells are slow to repair, to prolong the survival time of neutrophil function.