The Effects Of Low Concentration Of Metformin Combined With Gemcitabine On Aspc-1Cell Proliferation And Invasion And Its Possible Mechanism

ObjectiveTo study The effects of Low concentration of Metformin combined withGemcitabine on Aspc-1cell proliferation and invasion and its possible mechanism.MethodsThe reaction characteristics of Metformin in concentrations of0.01,0.02,0.04and0.08mM with or without Gemcitabine0.08μg/ml or Compound C10μmol/l werestudied by CCK-8assay and Transwell assay. Aspc-1without any Metformin andGemcitabine was used as control. Western blotting technique were used to detect thechanges of the total protein levels of AMPKα,p-AMPKα,ERK1/2and p-ERK1/2.ResultsThe use of Metformin in low concentrations only has on significant change onAspc-1cell proliferation and invasion. When combined with Gemcitabine, it hassignificant change, especially in Metformin0.08mM affiliated with Gemcitabine. Thelevel of p-AMPKα was significantly increased, while the level of p-ERK1/2has acorresponding reduction, especially in Metformin0.04and0.08mM affiliated withGemcitabine. After we added Compound C10μmol/l, the level of p-AMPKα wasdecreased, while the level of p-ERK1/2also has a significant reduction.ConclusionThe use of Metformin in low concentrations only has on significant effects onAspc-1cell proliferation and invasion. When combined with Gemcitabine, it caninhibit Aspc-1cell proliferation and invasion significantly. Apart from the AMPKpathway, the ERK pathway may be another important mechanism of the anti-tumoreffects of Metformin.