| Objective:：N-cadherin is one of ca2+dependent cadherin family, has importanteffects on proliferation、invasion and metastasis。Our previous paper has revealed thatN-cadherin down-regulation could enhance the metastatic potentials of HCC cell lineLM3cells in vitro, but do not be proved in vivo. So our present study is to investigateeffects of the down-regulated N-cadherin on metastatic potentials in nude mice modelorthotropic seeding with human HCC cells。Methods: We obtain five stable LM3cell lines： LM3-N2（lower N-cadherinexpression）， LM3-N6（normal N-cadherin expression），LM3-N7（weak N-cadherinexpression）and LM3-E2/LM3-E3（both are the control groups，normal N-cadherinexpression） from human hepatocellular carcinoma cell lines LM3by RNAinterference technology and testify it by western blot, then inject respectively the twocell clones (the LM3-N7cell clones and the control cell clones LM3-E2) into thelivers of BALB/c nude mice. After6weeks those mice are sacrificed, their lungs, livers and lymphonodus are infiltrated with formaldehyde, embedded with paraffin,and chemically stained by HE, aiming to observe metastasis sites and analyze theaffection of down-regulated N-cadherin on the metastasis potentials of humanhepatocellular carcinoma cell LM3.Results: The incidence rates of two cell clones about ascites, lung metastasis,intrahepatic metastasis and lymphonodus metastasis are (46.2%)6/13vs(0%)0/12,(46.2%)6/13vs(41.7%)5/12,(23.1%)3/13vs(16.7%)2/12,(61.5%)8/13vs(25%)3/12, byanatomic observation of nude mice and chemical staining of samples. The rates ofascites have significant difference by static analysis and others don’t.Conclusion. The down-regulated N-cadherin is in some extent effective in enhancingthe metastasis potentials of nude mice model orthotropic seeding with human HCCcell line LM3cells, and provides some theoretical basis for the subsequent research ofN-cadherin on metastasis potentials in hepatocellular carcinoma. |
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