The Association Study Of Respiratory Virus With Chronic Rhinosinusitis

ObjectiveTo determine whether respiratory virus could be detected in the ongoing inflammation of CRS by using polymerase chain reaction techniques and whether there is different in the virus species and to explore the function of the respiratory virus in CRS.Materials and MethodsThe control group consisted of27individuals (19men and8women), whose mean age was32.1years and ranging in the duration of symptoms from six day to more than ten years. The CRSsNP group according to established criteria consisted of30individuals (22men and8women), whose mean age was58.7years and ranging in the duration of symptoms from three months to more than twenty years. The CRSwNP group according to established criteria consisted of35individuals (25men and10women), whose mean age was37.4years and ranging in the duration of symptoms from six months to more than twenty years. In the CRSwNP group, there were10individuals with high eosinophil in hemogram and25individuals without high eosinophilic in hemogram. The epithelial cells were scraped in the endoscope room from the lateral side of the middle nasal meatus using a sterile Rhino-probe mucosal curette. Scrapings were immediately removed from the Rhino-probe cusp and were put into a plastic tube containing1mL of stroke-physiological saline solution. The respiratory virus was detected by using PCR techniques.ResultsIn92patients, a high proportion of specimens tested positive for virus (71.7%). But no significant difference in positive virus rates were identified among three groups. The most prevalent virus was RV, with33%positive rates, the next was Inf-A (20%), then Inf-B and PIV-1, both15%, RSV (13%), OC43(12%), PIV3(10%), the positive rates of PIV-2and 229E were both3%, but were detected only in control group. However, there was no different among three groups. In CRSwNP group, the positive rate of Inf-B is significant high in high eosinophil in hemogram group than without. The positive virus rates were high in the man patients than the female patients.Conclusion1. The total positive rates of respiratory virus were71.7%that were very high. Rhinovirus was by far the most prevalent virus. But there was not different among the three groups. It seems likely that the CRS patients are not more susceptible to virus infections.2. Mutiple positive virus rates account for more than50%total positive virus rates, suggesting us that multiple virus is very common in nasal mucosal, and a single fator of multiple virus infection may not induce diease.3. Among three groups, the potives virus rates were high in the man patients than woman, it seems that man is more susceptibility infection. But there is no more symptomatic infection.