MiR-21Suppresses Radiosensitivity In Cervical Cancer

Purpose:According to the radiation sensitivity of cervical cancer tissue, we screened out miR-21, then we studied the relation between miR-21expression and radiosensitivity in cervical cancer, and found the mechanism of miR-21regulating the radiosensitivity of cervical cancer.Methods and Materials:We obtained the miRNAs associated likely with radiosensitivity though screening miRNAs expression profiling in radiosensitive and radioresistant human cervical cancer sample using microarray. We chose the miR-21to the next experiment. Cervical cancer cell, SiHa and Me180, were transfected by miR-21-mimic, miR-21-inhibitor. Colony-Formation Assay was performed to detect the radiosensitivity. With agomir-21, and agomir-NC, we verified the radiation sensitivity of stably transfected lines in vivo. Through cell proliferation assay, cell cycle assay and apoposis assay, we searched for the molecular mechanism of miR-21depressed radiosensitiviy. Furthermore, three prediction algorithms PicTar, TargetScan and DianaLab were used to analyze the possible target gene.Results:The overexpression of miR-21could inhibit the radiosensisitivity of cervical cancer cell, while underexpression of miR-21can promote the radiosensitivity of cervical cancer cells. We used angomir overexpresing miR-21injected into nude mice tumors, the result showed miR-21could inhibit the radiosensitivity of cervical cancer in vivo. Furthermore, miR-21could promote cell proliferation, inhibit apoptosis, and did not affect the cell cycle distribution. Except for proliferation, cell cycle, apoptosis experiments, we also use bioinformatics softwareto predicted the target gene of miR-21, YAP became the candidate.Conclusions:We concluded that miR-21regulates radiosensitivity in cervical cancer through regulating apoptosis.