Preliminary Study On Chemical Analysis And Metabonomics Of Tibetan Medicine Sanguo Tang San

Tibetan medicine Sanguo Tang San as the basic preparation in Tibetan medicine, consisted with Terminalia chebula Retz., T.billerica (Gaertn.) Roxb. and Phyllanthus emblica L. Fructus Chebulae, which can clear the blood-heat and dissolve the bad blood. Sanguo Tang San, is used for the treatment of plateau diseases. Modern studies have found that plateau disease are caused by metabolic imbalances of free radicals imposed by plateau hypoxia and series of pathological changes whereby from oxidative stress induces apoptosis.Therefore, the blank SD rats were used for the research. Based metabonomics by GC-MS, HPLC analysis of plasma samples with which was intervened by san guo tang san.Objective:This paper presents a thorough study of Tibetan medicine Sanguo Tang San. Based on metabonomics, classic column chromatography, and the quantitative chemical analysis method, qualitative research on the the ethyl acetate fractions of terminaliae billericae by GC-MS, HPLC, TLC and other analytical methods; quantitative analysis on gallic and ellagic of Sanguo Tang San; preliminary study of Sanguo Tang San intervention in normal SD rats correlates; Analysis of Tibetan medicine Sanguo Tang San before and after the intervention of humoral metabolites (including drugs and their metabolites, endogenous metabolites) full spectrum dynamic change.Methods:1、Research on the ethyl acetate fractions of Terminalia billerica (Gaertn.)Roxb. by Silica gel column chromatography, polyamide column chromatography, preparative thin layer plant chemical separation method, Which posses good antioxidation, combined with1H-NMR,13C-NMR modern spectroscopic techniques to determine the structures of the compounds.2、Using high performance liquid chromatography (HPLC) to quantitative gallic and ellagic acid in Sanguo Tang San, which has strong antioxidant effect. 3、Study of the mechanism spectrum of free amino acids in blank rat plasma intervened by Tibetan medicine Sanguo Tang San.Results:1、To separate the ethyl acetate extracts of Terminalia billerica (Gaertn.)Roxb. by silica gel-column chromatography, gel-column chromatography, preparative TLC, etc. and identify with modern spectrum technologies of’H-NMR and13C-NMR.2、To determine gallic acidand ellagic acid, the stronger antioxidative ingredient, as the index component in Sanguo Tang San by HPLC.3、Combined with chemometric pattern recognition methods,can distinguish Sanguo tang for medicine group and blank control group rats very well.The6kinds of potential biomarker were found by the classification and characteristics.