| Chinese magnoliavine fruit, ripe fruit of Magnoliaceae plant of Schisandra chinensis(Turcz.) Baill,is one of Chinese herb which is used as medicine and food.So far,most study of Chinese magnoliavine fruit have been based on the things like lignans,which is defined as active component by dispensatory. The modern pharmacology research indicated that it has the good curative effect in many aspects,and the clinical practice is widespread.In this paper, the marked goods of schizandrol A and schisandrin A were made the maximum absorption wavelength detection in the methanol and ethanol. A RP-HPLC method for the simultaneous determination of schizandrol A and schisandrin A was established, and the determination was performed by RP-HPLC on AgilentTC-C18column(5μm,250mm×4.6mm)with methanol-H2O (75:25.V/V) as the mobile phase, the detection wavelength was selected at226nm, column temperature was30℃with the flow rate of moloile phase at0.7mL/min. Extractive methods includ ethanol refluent extract, ethanol seeping extract and ethanol ultrasonic extract of Schisandra chinensis(Turcz.)Baill with the schisandrol A and schisandrin A which were the essential active components in Schisandra chinensis(Turcz.)Baill as the test indicators.Results of the three methods,the contents of schisandrol A and schisandrin A extracted by ultrasonic extract were higher than the others.Ultimately, we choose this extraction conditions:the powder of Chinese magnoliavine fruit belowed40mesh was ultrasonic extracted2times according to solid-liquid ratio1:15by adding95%ethanol as solvent,30min for once, repeated two times, leaching, amalgamating the alcohol extract. Schizandrol A and schisandrin A obtained reach1.896mg/g and0.667mg/g. Compare the Combination of the enzyme treatment (the cellulase (4000U/g)0.1%, the pectinase (2000U/g)0.02%) and ultrasonic extraction method with a simple ultrasonic method, the extract of Schizandrol A and schisandrin A was substantial increase, almost2-3times.Through the comparison of steam distillation method, water ultrasonic wave-ethyl ether extraction, ethyl ether ultrasonic, ethyl ether microwave extraction, establishment steam distillation was established as the optimum method of extracting volatile oil. The essential oil extracted through this method is pure and transparent, without impurities. The joint extraction of essential oil and lignin such as schizandrol A and schisandrin A proved that extracting the essential oil did not destruct extracting other components of Chinese magnoliavine fruit and increasing the utilization of raw materials.The Schisandra alcohol extract acquired by Ultrasonic Extraction method and the edible ethyl alcohol made the different concentrations mixed liquor. After steam distillation for extracting the volatile oil, water extracts and the residue made Schisandra fermenting wine. And test and examine the content of schizandrol A and schisandrin A, alcohol content, total acid, volatile acid, total ester, light transmission rate, residual sugar, and other indicators. From the six kinds of clarifying agent,PVPP, pectinase,2%bentonite solution,1%chitosan,2%gelatin, activated carbon, we filter out1%chitosan as the best agent to clarify the mixed liquor to preserve more functional component and the color after processing droped fewer.The Fenton reaction and pyrogallol auto-oxidation method were used to test the ability of antioxidant capacity such as oxygen free radical elimination and hydroxyl free radical elimination about Schisandra extract, mixed liquor,fermented wine and etc. The extracting liquid by the enzyme treatment had the strongest capacity of Hydroxyl free radical scavenging,was approximately six times of the one without the enzyme treatment, while there is little change in the relative rate of superoxide scavenging of all samples. |
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