Potentiate Antitumor And Suppress Acute Nephrotoxicity Effects Of Ginseng Extract, Radix Astragali Extract, Acanthopanax Extract On Mlabris In Aidi Injection

Aim: This study was designed to research the potentiate antitumor and suppressacute nephrotoxicity effects of Ginseng extract, Radix Astragali extract,acanthopanaxextract on Mlabris in Aidi injection.Methods: MTT essay was used to observe the effect of Ginseng extract, RadixAstragali extract, acanthopanax extract on cytotoxicity caused by Mlabris on cells（SMMC-7721human hepatocellular cells, Hela human cervical carcinoma cells, U251human glioma cells, NRK rat kidney cells）. The change of cell morphology inSMMC-7721cells and NRK cells was used to determine the potentiate antitumor andsuppress acute nephrotoxicity effects. We investigated the effects of Ginseng extract,Radix Astragali extract, acanthopanax extract on inhibitory rates induced by Mlabris inmice transplantad with H₂₂cell. The protective effects of Ginseng extract, RadixAstragali extract, acanthopanax extract on renal injury caused by Mlabris in rats wereprojected. Then we study the change of Na⁺K⁺-ATP enzyme, Ca²⁺Mg²⁺-ATP enzyme,LDH enzyme for SMMC-7721cells and NRK cells; Flow cytometry was used toanalyze the apoptosis rate of SMMC-7721and NRK cells; the morphological changeseffect of drug-induced apoptosis on SMMC-7721observed with Hoechst33342staining;We observed the renal micro-structural changes under the electron microscopyrespectively; western blot was employed to determine alternations of bcl-2/caspase-3levels in SMMC-7721and NRK cells.Results: Ginseng extract, Radix Astragali extract, acanthopanax extract couldenhance inhibitional proliferation induced by Mlabris on SMMC-7721; Ginseng extract（0.66,1.33mg/kg）, Radix Astragali （0.28,0.55mg/kg） extract, acanthopanax extract （0.53,1.05mg/kg） could enhance the ati-tumor effects of Mlabris in transplantabletumor models of H22（q>0.85）; Ginseng extract （385.00,770.00μg/ml）, Radix Astragali（32μg/ml） extract could gradually increase the elevation of LDH activities induced byMlabris （1.25μg/ml）（p<0.05） on SMMC-7721; Meanwhile Ginseng extract（385.00,770.00μg/ml） could also promote the decreasing of Na⁺K⁺-ATP/Ca2+Mg2+-ATPactivities induced by Mlabris （1.25μg/ml） on SMMC-7721; The result of flowcytometry showed that Ginseng extract （385.00,770.00μg/ml）, Radix Astragali （32.00μg/ml） extract, acanthopanax extract （76.50μg/ml） could improve the apoptosis rateinduced by Mlabris （1.25μg/ml） on SMMC-7721cells; significantly we found thatGinseng extract （770.00μg/ml）, Radix Astragali （32.00μg/ml） extract could change theexpression of bcl-2/caspase-3induced by Mlabris （1.25μg/ml） on SMMC-7721cells.The viability of NRK cells exposed to Mlabris （0.31-5.00μg/ml） singnificantlydecreased; Ginseng extract （24.25,48.5μg/ml）,Radix Astragali extract （4.00,8.00μg/ml）, acanthopanax extract（9.56μg/ml） could reduce cytotoxicity induced byMlabris （1.25μg/ml） on NRK cells; meanwhile Ginseng extract （1.59,3.19mg/kg）,Radix Astragali extract （0.66,1.32mg/kg）, Acanthopanax extract （1.58,3.16mg/kg）could decrease the contents of blood urea nitrogen, serum creatinine and proteinuriainduced by Mlabris（0.04mg/kg） in rats; Ginseng extract （24.25,48.5μg/ml）, RadixAstragali（4.00μg/ml） extract, acanthopanax extract （9.56μg/ml） not only couldgradually decrease the elevation of LDH activities induced by Mlabris （1.25μg/ml）（p<0.05）, but also significantly increase the reduction of ATP enzyme activities inducedby Mlabris （1.25μg/ml）（p<0.05） on NRK cells; Ginseng extract （24.25,48.5μg/ml）,Radix Astragali（4.00,8.00μg/ml） extract, acanthopanax extract（9.56,19.13μg/ml）could inhibit the apoptosis rate induced by Mlabris （1.25μg/ml） on NRK cells;then we found that Ginseng extract （24.25μg/ml）, Radix Astragali （4.00μg/ml） extractcould change the expression of Bcl-2/Caspase-3induced by Mlabris （1.25μg/ml） onNRK cells.Conclusion: Ginseng extract, Radix Astragali extract, acanthopanax extract haveefficiency potentiate antitumor and suppress acute nephrotoxicity effects, and itsmechanism may be related to enhancing the expression of caspase-3（bcl-2） induced by Mlabris on SMMC-7721（NRK） cells, and be related to reducing the expression of bcl-2（caspase-3） induced by Mlabris on SMMC-7721（NRK） cells,...