Study On Repair And Regeneration Of Peripheral Nerve Anastomosis After Inject Mouse Nerve Growth Factor Into Sheath

OBJECTIVE:TO observe the repair and regeneration of nerve growth factor on rabbit sciatic nerve anastomosis, And compare the treatment methods difference of rabbit sciatic nerve repair and regeneration after anastomosis between intrathecal and local application of NGF。METHODS:The24New Zealand white rabbits were randomly divided into A, B, C, D four groups (n=6).Suturing the rabbit sciatic nerve after cut-off,The group A received intrathecal injection of NGF, while group B was given by local injection of NGF. The group C received intrathecal injection of saline, and the group D was given by local injection of saline.To observe the changes in general and histological under light microscope, and to detect electrophysiological and immunohistochemical changes in12weeks after operation.RESULTS:After12weeks, intrathecal application of NGF group,compared with the other three groups, has complete sheath nerve fibers, sheath thickened, orderly arranged, well regenerated, and little difference with the normal nerve fibers; Intrathecal application of nerve growth factor in neural stem conduction velocity, myelinated nerve fiber count, thickness of myelin sheath were superior to the other three groups with significant difference (P<0.05). The comparison between topical application of nerve growth factor and application of NS has significant difference in neural stem conduction velocity, myelinated nerve fiber count, and myelin thickness (P<0.05). CONCLUSION:NGF has effected in promoting peripheral nerve repair and regeneration after anastomosis. Intrathecal application of NGF is superior to the topical application。...