The Effects Of Aerobic Exercise And Konjac Glucomannan On Resistin And AMPK During The Formation Of Hepatic Insulin Resistance In SD Rats

Objective:To explore the effect of aerobic exercise and Konjac glucomannan on resistin and AMPK during the formation of rat hepatic insulin resistance and its mechanism.Method:50male SD rats which were6week old and weighs180to220grams were selected. One week of adaptive feeding, then they were randomly divided into5groups, control group (C group, n=10), high-fat and high-carbohydrate group (HF group, n=10), high-fat and high-arbohydrate with Konjac glucomannan Group (HF+KGM groups, HK, n=10), high-fat and high-arbohydrate with exercise training group (HF+E group, HE, n=10), high-fat and high-arbohydrate with exercise and Konjac glucomannan Group (HF+E+KGM Group, HEK, n=10). The C group were fed with ordinary diet, while the other4groups were all fed with high-fat and high-arbohydrate diet. At the same time, the HF group didn’t take exercise training, the HK group added KGM, the HE group took exercise training, and the HEK group added KGM and took exercise training; Exercise training were used60minutes’ unloaded swimming for11weeks,6times per week; the adding dosage of KGM was50mg/kg weight.11weeks later, drew materials on next morning of the last exercise training and keeping fasting. Serum TG、FPG、FINS and hepatic resistin、PEPCK、G-6-Pase concent in each group of rats were examined.Results:①After11weeks of high-fat and high-carbohydrate diet, compared with C group, the hepatic PEPCK and G-6-Pase contents increased significantly in HF group rats, meanwhile, serum FPG、FINS contents and HOMA-IR increased significantly (P<0.01)②Through the double factor variance analysis, aerobic exercise could significantly lower hepatic PEPCK (P<0.05) and G-6-Pase (P<0.01) contents in rats with high-fat and high-carbohydrate diet, moreover significantly lower serum TG (P<0.01)、FPG (P<0.05). FINS (P<0.01) contents and HOMA-IR(P<0.01). KGM could significantly lower hepatic PEPCK(P<0.01)and G-6-Pase (P<0.01) contents in rats with high-fat and high-carbohydrate diet, and significantly lower FPG (P<0.05)、FINS (P<0.01) contents and HOMA-IR (P<0.01), but serum TG was no significant difference. Aerobic exercise combined with KGM had a significant interaction for decreasing hepatic PEPCK (P<0.01) and G-6-Pase (P<0.05) contents in rats with high-fat and high-carbohydrate diet; Although aerobic exercise combined with KGM could make FPG level in rats with high-fat and high-carbohydrate diet to be further decreased (P>0.05), it was no significant difference, while that had significant interaction for TG (P<0.01)、FINS (P<0.01) and HOMA-IR (P<0.05), could make TG、FINS and HOMA-IR to be further decreased.③After11weeks of high-fat and high-carbohydrate diet, compared with C group, hepatic AMPK and resistin in HF group of rats were significantly increased (P<0.01). The double factor variance analysis showed that aerobic exercise could significantly reduced hepatic resistin (P <0.05) and AMPK levels (P<0.05) in rats with high-fat and high-carbohydrate diet; Although the Konjac glucomannan supplement could reduce hepatic resistin content in rats with high-fat and high-carbohydrate, there was no significant differences. but it could significantly reduce hepatic AMPK levels in rats with high-fat and high-carbohydrate diet(P<0.01). Aerobic exercise combined with Konjac glucomannan supplement had significant interactions for reducing heapatic resistin levels (P<0.05) and AMPK levels (P<0.01) in high-fat and high-carbohydrate diet rats.Conclusions:①11weeks of high-fat and high-carbohydrate diet could significantly increase hepatic PEPC、G-6-Pase contents and HOMA-IR in rats, which showed11weeks of high-fat and high-carbohydrate diet could induce the form of hepatic insulin resistance in rats.②Aerobic exercise or KGM could significantly prevent the form of hepatic insulin resistance in rats induced by high-fat and high-carbohydrate diet, leading to the reduction of hepatic PEPCK. G-6-Pase contents and HOMA-IR in rats with high-fat and high-carbohydrate diet. Moreover, aerobic exercise combined with KGM could prevent the form of hepatic insulin resistance induced by high-fat and high-carbohydrate diet in rats, and had a significant promotion to a certain exent.③Increasing of serum lipids and hepatic resistin levels in rats with high-fat and high carbohydrate diet might be related to the formation of hepatic insulin resistance, and exercise training or Konjac glucomannan supplement could prevent the formation of hepatic insulin resistance by improving blood lipid metabolism and reducing the hepatic resistin content.