Study On CIA Rat’s Inflammatory Reaction Affected By Uc-MSC With Superparamagnetic Nanoparticles

Objective:This subject selects SD rats to establish a rheumatoid arthritis (CIA) model, and the accumulation of Uc-MSC in CIA joint and its impact on inflammation network was observed by using Uc-MSC with superparamagnetic iron oxide (SPIO), through MRI imaging approach to study the theraputic effect of umbilical cord mesenchymal stem cells (Uc-MSC) on the inflammatory pathological processes of rheumatoid arthritis (RA).Subjects:CIA ratsMethods:CIA rat was established by Type II collagen and Complete Freund’s Adjuvant (CFA) jointly. Totally50SD rats were divided into5groups: Uc-MSC group, superparamagnetic iron oxide (SPIO) group and superparamagnetic iron oxide combined with Uc-MSC (SPIO-UCMSC) group (each group was injected into intravenously via tail vien,0.5milliliter), as well as model group and normal group.10rats in each group were randomly selected. The AIs of the rats were evaluated weekly. Each group was taken on MRI imaging after5weeks. Blood was collected through abdominal vien one week or five weeks after treatment.3ml blood wae uesd for serum separation and further enzyme-linked immunosorbent assay (ELISA) test of IL-1(interleukin-1, IL-1), IL-6, IL-18, TNF-a (Tumor necrosis factor-a), vascular cell adhesion molecule (VCAM-1) levels;2ml blood was added into heparin tube and then determined cell surface CD11b through Flow Cytometry (FCM). Another method was also carried out for the routine pathological examination of knee joint synovial hyperplasia resected from the sacrificed rats.Results:1. The AI evaluate, joint X-ray film and the synovial tissue pathology indicate that CIA rat model is successfully established by using Type Ⅱ collagen and Complete Freund’s Adjuvant.2. The serum IL-1, IL-6, IL-18, TNF-α, VCAM-1and neutrophil cell surface expression of CD11b of CIA model group increase obviously (P<0.05), but all these cytokine and cell surface marker decreae evidently (P<0.05) after Uc-MSC treatment, and the AI evaluate also decreae.3. The serum levels of inflammatory factors after5weeks treatment of SPIO-UcMSC and Uc-MSC are less than those after1week treatment and model group’s (P<0.05). The MRI imaging of CIA rat’s lower limb is clear for SPIO-UcMSC group.4. Compared SPIO-UcMSC group with Uc-MSC group, the serum levels of inflammatory factors is similar, and the result indicate indifference between both of them (P>0.05).Conclusions:1. Release of cytokines that of inflammatory network are obviousely upregulated during the pathogennsis process in CIA rats. The upregulation of these cytokines is involved in the formation of synovial lesions and immunologic arthritis in RA.2. SPIO-UcMSC can uptake in the lesion joint and imaging clearly under MRI, which indicates umbilical cord stem cells possess chemotaxis that is able to gather to inflammatory injury site.3. After the treatments by Uc-MSC, the serum IL-1, IL-6, IL-18, TNF-α, VCAM-1levels and neutrophil cell surface expression of CD11b in CIA rats is evidently lower than model group’s, and the AI evaluate is improved. These obtained results indicate that Uc-MSC can inhibit from the release of the inflammatory factors and improve the immune inflammatory reation in RA.