A Cell-Based Artificial Antigen-Presenting Cell K32/4-IBBL/CD86for Stimulation And Amplification Of Human T Cells Of HCC

Objective:To study the effects of A Cell-Based Artificial Antigen-Presenting Cell K32/4-IBBL/CD86for stimulation and activation of human T cells of HCC.Methods:We accomplished the artificial antigen-presenting cells by coupling K32/CD86/4-IBBL cell with OKT3monoclonal antibody against CD3, named K32/CD86/4-IBBL/OKT3cells. Testing CD in CD4+T cells and the ability of secreting IFN-yin activated CD4+T cells by FACS. Testing the ability of secreting IFN-yin activated CD8+T cells by FACS. Testing the killing function of CTL upon BEL7402by MTT method.Results:The activation state, proliferation and the change of cell phenotypes the ability to secreteIFN-γ. From the experimental results we have found that CD are down-regulated observably in CD8+T cells compared with the control groups (P<0.05); The irradiated cells were added to the culture three times at days1,8,15, respectively; The proliferation ability of activated CD8+T cells increase3times than of the control group; the ability of secreting IFN-γ in activated CD8+T cells is high than in control groups, compared with CD8+T cells alone (P<0.05). the activation state, proliferation and the change of cell phenotypes the ability to secrete IFN-γ. From the experimental results we have found that CD are down-regulated observably in CD4+T cells compared with the control groups (P<0.05); The ability of secretingIFN-γ in activated CD4+T cells is high than in control groups.(P<0.05). In clinical immunotherapy we likely require cells with antigen-specific cytolytic functions which were. we selected HLA-A2positive HCC cancer cell line (BEL7402) as target cell. Testing the killing function upon BEL7402by MTT method. when the effect/target rates are at50:1、10:1、1:1,the killing rates are. From the experimental results we have found that The killing functions of CTL upon BEL7402are remarkable compared with the control groups (P<0.05).Conclusion:A Cell-Based Artificial Antigen-Presenting Cell K32/4-IBBL/CD86can stimulate and activate of human T cells of HCC.