| Objective:To pour various doses of alcohol into rats by gastric lavage within specified periods of time to observe their changes in the heart structure and function, detect the changes of ACE-. ACE2and AngⅡ in their myocardial tissue and explore the mechanism of alcohol damage to the heart.Methods:According to three different periods of time, namely,4W,8W and12W respec-tively,124SD rats were randomly divided into Saline Group, Alcohol Group, Liquor Group, each of which were further divided into low-dose group(0.25ml/100g/d), middle-dose group (0.5ml/100g/d) and high-dose group(lml/100g/d), a total of27groups. Rats were given liqu-or、alcohol and sline orally twice a day (each time half of their daily specified dose), respe-ctively, with an interval being more than9h. Their drinking amounts were adjusted in accor-dance with their weight weekly. Left ventricular pressure was determined, indexes of HW BW were calcul-ated. myocardial fibrosis was observed by Masson staining, Angll, ACE, ACE2content of myocardial tissue were detected by ELISA, ACEmRNA and ACE2mRNA expression by RT-PCR, ACE and ACE2expression by immunohistochemicals of myocar-dial cells.Results:①Compared with Saline Group, there were significant change(P<0.05) in left ventricular pressure and HW/BW index of both Liquor Group and Alcohol Group of any dose in12W.②The myocardial cell mildly went turbid and degenerated, and the fibrous tissue showed a mild tendency of hyperplasia in middle-dose group and high-dose group of Liquor Group and Alcohol Group in4W. With the dose increased and time prolonged, the myocardial cell gradually became turbid, degenerative and the fibrous tissue gradually became hyperplasia, there were obvious changes in alcohol groups of any dose in8W^12W. but hyperplasia worsened in the adipose tissue in high-dose groups in12W, there were obvious changes in alcohol groups of any dose.③The Angll and ACE contents of the myo- cardial tissue of both Liquor Group and Alcohol Group of middle and high doses are higher than the saline control group、low-dose groups(P<0.05, P<0.01) in8W, the AngⅡ and ACE contents maintained higher level in middle and high dose groups, but in the high-dose alcohol group, the Angll content was higher than that of the middle-dose alcohol group (P<0.05) in8W. Their in both Liquor Group and Alcohol Group of any dose were respec-tively higher than that of Saline Group in12W (P<0.05, P<0.01, P<0.01). Furthermore, the Angll and ACE contents maintained high level in both the middle and the high dose groups, higher than that of the low-dose group (P<0.05), but in Liquor Group of high-dose, the Angll content was higher than that of Liquor Group of middle-dose (P<0.05).④he ACE2contents in the myocardial tissue of Liquor Group and Alcohol Group of any dose were higher than that of Saline Group in8W (P<0.05, P<0.01, P<0.01), while the ACE2contents in both the middle-dose group and the high-dose group were higher than that of the low-dose group (P<0.05, P<0.01) and the ACE2content of the high-dose group was higher than that of the middle-dose dose(P<0.05).In12W, the ACE2contents of Liquor Group and Alcohol Group of low dose were significantly higher than that of Saline Group (P<0.01), while ACE2levels in Liquor Group and Alcohol Group of middle dose and high dose were respectively lower than that of Saline Groupand low dose in12W (P<0.01,P<0.01).⑤In the middle-dose group and the high-dose group of Liquor Group and Alcohol Group the ACE2mRNA expressions in the myocardial tissue were higher than that of Saline Group (P<0.05), higher than that of the low-dose group (P<0.Ol).In12W, the ACE2mRNA expre-ssion in low dose of Liquor Group and Alcohol Group were higher than that of Saline Group (P<0.01), while the ACE2mRNA expressions in both the middle-dose and the high-dose groups were respectively lower than that of Saline Group and low dose(P<0.05, P<0.01).⑥In the middle-dose group and the high-dose group, the ACEmRNA expressions in the myocardial tissue were respectively higher than that of Saline Group in8W(P<0.05, P<0.01). In the high-dose group, the ACEmRNA expressions were respectively higher than those of the middle-dose groups and the low-dose groups in8W (P<0.05, P<0.01). In12W, the ACEmRNA expressions in Liquor Group and Alcohol Group of any dose were respec-tively higher than that of Saline Group (P<0.05, P<0.01, P<0.01) while the ACEmRNA expressions in middle-dose groups and high-dose groups were respectively higher than those of low-dose groups (P<0.05, P<0,01) and in high-dose groups, the ACEmRNA expressions were higher than those of middle-dose groups (P<0.05).Conclusion:①small amount of alcohol consumption do not do apparent damage to the myocardial function in short term.②Long-term drinking can lead to myocardial fibrosis, whose degree is related to of the amount of alcohol consumption and drinking history.③Long-term alcohol consumption can damage the balance of cardiac ACE and ACE2, causing the increase of ACE and Angll and the decrease of ACE2, resulting in myocardial fibrosis.④Under the same conditions, pure alcohol is more obvious changes than alcohol consump-tion on the heart. |
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