Expression And Significance Of Notch Receptors And Ligande In Primary Hepatocellular Carcinoma

Objective To investigate the expression of Notch receptors (Notch-1,Notch-2) and ligands (Jagged-1,D11-1) in the primary hepatocellular carcinoma and healthy donor normal liver tissues and cells.Explore the variability of these four kinds of proteins’respective expression between Primary hepatocellular carcinoma and healthy donor normal liver tissues and cells.Investigate the role of Notch receptors, ligands in the development of primary hepatocellular carcinoma and its clinical significance.Methods The specimens of acceptor and donor liver tissues were collected when the patients undergone OLT in the Institute of Liver Transplantation of the general hospital of Chinese People’s Armed Police Forces. The specimens were selected and divided into two groups:the first group contains30cases of primary hepatic carcinoma tissue and the second group have30cases of normal liver tissue.The expression of Notch receptors (Notch-1,2) and ligands (Jagged-1, D11-1) in this two group tissues were detected by immunohistochemistry (PV two-steps). To analysis the expression differences,correlation and contact with the clinical data by the Non-parametric test and Spearman correlation analysis.Results The expression of Notch-1located in the cytoplasm and nucleus of liver cells. In PHC tissues and cells, the positive rate was86.67%(26/30), while the positive rate was40%(12/30) in healthy donor normal liver tissue and cells and cells. The expression of Notch-1in PHC tissues and cells was higher than in healthy donor normal liver (Z=-3.719, P=0.000);The expression of Notch-2located in the cytoplasm of liver cells. In PHC tissues and cells, the positive rate was73.33%(22/30), while the positive rate was63.33%(19/30) in normal donor liver tissues and cells. The expression of Notch-2has no significant difference between PHC and healthy donor normal liver tissues (Z=-1.115,P=0.265); Jagged-1was expressed in the cytoplasm of liver cells.In PHC tissues and cells, the positive rate was76.67%(23/30), while the positive rate was46.67%(14/30) in healthy donor normal liver tissues and cells.The expression of Jagged-1in PHC tissues and cells was higher than in healthy donor normal liver tissues (Z=-3.369,P=0.001). The expression of D11-1located in the cytoplasm of liver cell.In PHC tissues and cells, the positive rate was70.00%(21/30), while the positive rate was53.33%(16/30) in normal donor liver tissues and cells. The expression of Dll-1has no significant difference between PHC and normal donor tissues and cells (Z=-0.041, P=0.963); Analyse the correlation between the expression of Notch-1proteinum in PHC tissues and Jagged-1:the expression of Notch-1in PHC tissues has low positive correlation with Jagged-1(rs=0.379, P=0.039).In PHC tissues,there have no significant dependablity between Notch-1,Jagged-land age, AFP,differentiation,diameter of tissues.(rs=0.308,P=0.098;rs=0.018,P=0.926;rs=0.162、 P=0.393;rs=0.011,P=0.954respectively and r,=-0.116, P=0.543;rs=-0.219,P=0.303; rs=0.326,P=0.078;rs=0.201,P=0.288respectively)Conclusion Notch receptors (Notch-1,2) and ligands (Jagged-1, Dll-1) were expressed in primary hepatocellular carcinoma tissues and healthy donor normal tissues.In healthy donor normal tissues, The expression levels of Notch receptors (Notch-1,2) and ligands (Jagged-1, Dll-1) in normal liver tissues participation to maintain the function and form of healthy normal donor tissues.Therefore, Notch-1and Jagged-1were more meaningful for the reasearch of primary hepatocellular carcinoma than others.In PHC tissues, there was positive correlation between the expression of Notch-1and Jagged-1, but it is not strong. This shows that Notch-1and Jagged-1are not only synergies but also independently the affects of occurrence and development of PHC.