The Directional Plerosis On Rabbit Liver Ischemia-reperfusion Injury By Bone Marrow Mesenchymal Stem Cells

ObjectiveTo study the repair with Bone Marrow Mesenchymal stem C ells(BMSCs) on hepatic ischemia reperfusion injury in rabbits,and compare withthe effect of ischemic preconditioning (Ischemia preconditioning, IP), anddiscuss its mechanism.MethodGet1health New Zealand rabbits which is4weeks old, take the BoneMarrow Mesenchymal stem cell suspension，culture and amplification to thethird generation, some of them use to analysis the expression of CD14, CD44antigen on BMSCs surface by flow cytometry, the left are all fortransplantation.Get32health New Zealand rabbits which are8-12weeks olddivided into:group A(ischemia reperfusion),group B(bone marrowmesenchymal stem cell suspension transplantation after ischemia reperfusion),group C(ischemia preconditioning), group D(sham operation) randomly. Rabbitsof group A are undertaken median incision, exposure the hepatic portal, clampthe hepatic portal with scatheless artery clamp, remove the artery clamp andimplement reperfusion after occlude the hepatic blood flow30min completely,reperfusion lasts30min. Rabbits of group B are injected0.5ml1x106/mlBMSC suspension on internal jugular vein slowly after ischemia reperfusion, theleft operation just like group A. Group C, occluding the hepatic portal which makes hepatic ischemia and lasts5min, then open the flow5min, repeat theoperation3times, the left operation just like group A besides above. Group D issham operation group. After the operation, close the opening abdomen, feedone week. Take liver tissue and blood after execute all the rabbits. Detecting theapoptosis index(AI) with TUNEL method.ResultsCultured BMSCs are proliferation, and with high purity, good stability andheterogeneity.②The serum levels of AST, LDH and apoptosis index (AI) of A,B, C group are higher than those in group D, and SOD level of the liver tissuewas lower than that in group A, the damage expressed on histopathology of liveris significantly worse than group D;the serum level of AST, LDH and apoptosisindex(AI) was lower than group A, group B are the lowest, and SOD level of livertissue in group B,C are higher than those in group A, B group are the highest,the damage expressed on histological in group B,C was much lighter than thatin group A, group B is the lightest. Differences were statistically significant(P <0.01).ConclusionBMSCs transplantation, play a part in repair in New Zealand white rabbitsliver ischemia reperfusion injury and the effect is better than pretreatment.