| ObjectiveSurgical coronary artery ligation method to establish rat model ofmyocardial ischemia-reperfusion injury, then to observe the protective effect ofradix morindae officinalis(RMO) pretreated on myocardial ofischemia-reperfusion injury, and explore the possible protective mechanismsof RMO during the process.Methods（1）80male rats were randomly divided into four groups equally: calledsham ligation (sham) group，myocardial I/R model group, high-dose3g/(kg·d)and low-dose1.2g/(kg·d) RMO groups. The rats in RMO groups the correspon-ding concentration of liquid gavage, rats in other two groups received a samevolume of Saline, there was no limittiong on drinkingwater, myocardial ischemi-a and reperfusion mode was settled after7days. Then CK-MB, Na+-K+-ATP-ase,Ca2+-Mg2+-ATPase,NO and INOS were measured, the change of myocar-dial ultrastructure (mitochondria, myofilaments Sarcolemma) and infarct sizewere observed in electron microscopy.ResultsCompared with Sham group, The level of CK-MB and iNOS in I/R groupwas significantly increased (P<0.05),and the ultrastructure of myocardial cellsobviously changed, the activities of NO and ATPase were significantlyreduced (P<0.05); Compared with I/R group, the activities of CK-MB and iNOSand the size of infarction were reduced (P<0.05), the degree of change in ultrastructure of myocardial cells was lighter; Compared with RMO1.2g/㎏group, the activity of CK-MB and iNOS and the size of infarction weresignificantly reduced (P<0.05) in RMO3.0g/㎏group, but the activity of NOand ATPase were significantly higher (P<0.05), and the degree of change inultrastructure of myocardial cells was obviously lighter.ConclusionRadix morindae offieinalls can protect rats from ischemic reperfusioninjury and reduce infarct size through reducing intracellular calciumoverload and accumulation of oxygen free radicals, and the role was dose-related. |
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